{"id":"W1972171258","doi":"10.1016/j.jchromb.2012.05.039","title":"Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC)","year":2012,"lang":"en","type":"article","venue":"Journal of Chromatography B","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":false,"ca_institutions":"Natrix Separations (Canada); University of Waterloo","funders":"","keywords":"Chemistry; Chromatography; Recombinant DNA; Cross-flow filtration; Membrane; Escherichia coli; Size-exclusion chromatography; Downstream processing; Ion chromatography; Bioreactor; Ion exchange; Protein purification; Affinity chromatography; Microfiltration; Penicillin amidase; Filtration (mathematics); Biochemistry; Enzyme; Ion; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007783175,0.0001861098,0.000316044,0.0001888719,0.00008835645,0.00002784787,0.0001391994,0.0002479496,0.00001432374],"category_scores_gemma":[0.0001590992,0.0001683749,0.0001752054,0.0003410996,0.0002176401,0.00006386424,0.00002501186,0.0001501299,1.564975e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009823532,"about_ca_system_score_gemma":0.00006691091,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001204781,"about_ca_topic_score_gemma":0.000004170741,"domain_scores_codex":[0.9983904,0.0001986292,0.0007116923,0.0002302233,0.0002854344,0.0001836737],"domain_scores_gemma":[0.9980246,0.00002488298,0.0009938428,0.0003092925,0.0004988611,0.0001485618],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001968537,0.0003271619,0.007152128,0.0002619745,0.0001150791,6.723901e-7,0.0005854191,0.00002837492,0.9896194,0.00005413166,0.00002161933,0.001637172],"study_design_scores_gemma":[0.0008400729,0.000379679,0.01941161,0.00005711035,0.0001537685,0.00007760279,0.0002537886,0.0007837387,0.9758121,0.00003868565,0.002001049,0.0001907573],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9742423,0.01792937,0.007164945,0.00005559938,0.0001179071,0.0004175779,0.00004101966,0.000006412447,0.00002484895],"genre_scores_gemma":[0.9862221,0.001954546,0.01154719,0.00001543207,0.0001699169,0.000007987677,0.00005938952,0.00001721409,0.000006188437],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01597483,"threshold_uncertainty_score":0.6866129,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01319237448746715,"score_gpt":0.2467138442384509,"score_spread":0.2335214697509838,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}