{"id":"W1972201556","doi":"10.1021/bi7010459","title":"Metal Requirements and Phosphodiesterase Activity of tRNase Z Enzymes","year":2007,"lang":"en","type":"article","venue":"Biochemistry","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Phosphodiester bond; Chemistry; Enzyme; Metal ions in aqueous solution; Hydrolysis; Stereochemistry; Endonuclease; Cleavage (geology); Transfer RNA; Metal; Enzyme kinetics; Biochemistry; RNA; Active site; Biology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001294577,0.0004236862,0.0003276074,0.0002696915,0.0001044241,0.0005016979,0.0003083107,0.000317852,0.0008193943],"category_scores_gemma":[0.0003483571,0.0001847081,0.0002111631,0.0002286778,0.0001732172,0.000219719,0.000232091,0.00035071,0.0003778549],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002604178,"about_ca_system_score_gemma":0.0001111727,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001036308,"about_ca_topic_score_gemma":0.001102475,"domain_scores_codex":[0.9997267,0.00004602916,0.00003623878,0.00006546825,0.00008296865,0.00004264015],"domain_scores_gemma":[0.999765,0.00005422466,0.00008874022,0.0000208601,0.00002848645,0.00004266611],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004998785,0.000005160674,0.000491747,0.00001525385,0.00000471435,0.00003067144,0.000007334132,0.00004085675,0.9988467,0.00001474247,0.000005624851,0.0004871363],"study_design_scores_gemma":[0.000008161845,0.0001416775,0.01527721,0.000004460507,0.00002074915,0.0006055462,0.00004120712,0.0008011443,0.9820448,0.0000470882,0.0009978393,0.0000101344],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9970285,0.000817532,0.001456665,0.00004016643,0.000005237348,0.000005180865,0.0002781546,0.00004793073,0.0003206496],"genre_scores_gemma":[0.9964339,0.000283066,0.001780542,0.00001605427,0.000003270413,0.000007438269,0.0009221444,0.00002772147,0.0005259108],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001036308,"threshold_uncertainty_score":0.002741098,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01457682738987434,"score_gpt":0.2653574675514831,"score_spread":0.2507806401616088,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}