{"id":"W1972800518","doi":"10.1089/hgtb.2013.155","title":"AAV2/8 Vectors Purified from Culture Medium with a Simple and Rapid Protocol Transduce Murine Liver, Muscle, and Retina Efficiently","year":2013,"lang":"en","type":"article","venue":"Human Gene Therapy Methods","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institute of Genetics; Fondazione Telethon","keywords":"Lysis; Centrifugation; Biology; Ultracentrifuge; Cell culture; Differential centrifugation; Extracellular; Transduction (biophysics); Cell; Molecular biology; Cell biology; Biochemistry; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005042756,0.001140673,0.0005296362,0.0007329143,0.0005276662,0.000574644,0.0005789969,0.0005781003,0.001741055],"category_scores_gemma":[0.0003422776,0.0003785621,0.0004583935,0.0002594859,0.0004748459,0.0004174371,0.0005534384,0.001563223,0.002177421],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003046502,"about_ca_system_score_gemma":0.0004425499,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006070152,"about_ca_topic_score_gemma":0.001119044,"domain_scores_codex":[0.9991686,0.0001135324,0.0001210424,0.0001831523,0.0003010677,0.0001125566],"domain_scores_gemma":[0.9997527,0.00003491521,0.00005721736,0.00004678012,0.00005668769,0.00005160862],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000311173,0.00003054272,0.000103551,0.00004444524,0.000004196006,0.00003332282,0.00002754871,0.00003509911,0.9973931,0.0001374368,0.0001707371,0.001988751],"study_design_scores_gemma":[0.0000184784,0.0002829561,0.001106736,0.00001919937,0.00002229771,0.0004233867,0.00001721033,0.000691587,0.9806665,0.0000939118,0.0166377,0.00002004852],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6786531,0.005440766,0.2951337,0.0006098544,0.0005368876,0.00269187,0.003840666,0.004058565,0.009034586],"genre_scores_gemma":[0.633441,0.0053598,0.3188961,0.0004708858,0.0001978387,0.00330055,0.01398055,0.001181216,0.02317204],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001741055,"threshold_uncertainty_score":0.005824387,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03325294322401454,"score_gpt":0.3559053005040341,"score_spread":0.3226523572800195,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}