{"id":"W1973150788","doi":"10.1016/j.diagmicrobio.2015.04.005","title":"Assay for estimating total bacterial load: relative qPCR normalisation of bacterial load with associated clinical implications","year":2015,"lang":"en","type":"article","venue":"Diagnostic Microbiology and Infectious Disease","topic":"Bacterial Identification and Susceptibility Testing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University; Jewish General Hospital","funders":"Jewish General Hospital; McGill University","keywords":"16S ribosomal RNA; Biology; Amplicon; Real-time polymerase chain reaction; Bacteria; Microbiology; Enumeration; Computational biology; Polymerase chain reaction; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.01595271,0.003915019,0.00276514,0.003678967,0.001372489,0.003491112,0.002354034,0.004530487,0.006389738],"category_scores_gemma":[0.01950528,0.002794245,0.00193804,0.002748112,0.004369749,0.001986923,0.002156982,0.006472033,0.005919287],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001863808,"about_ca_system_score_gemma":0.002316798,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001289924,"about_ca_topic_score_gemma":0.002414779,"domain_scores_codex":[0.9682311,0.007873598,0.001887916,0.006640809,0.01418833,0.001178209],"domain_scores_gemma":[0.9867025,0.005060393,0.001579317,0.002070042,0.004201056,0.0003866905],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008122747,0.000725512,0.01733498,0.001083478,0.0001989216,0.0001116694,0.0009513858,0.001347446,0.9245809,0.003398088,0.002720141,0.04673523],"study_design_scores_gemma":[0.00008721605,0.001437093,0.0177175,0.0003308582,0.0002815358,0.001105892,0.0003688606,0.01959695,0.9442418,0.003425356,0.01117174,0.0002352624],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.09698585,0.003143847,0.8748968,0.001110401,0.001763089,0.002372685,0.004011571,0.004452509,0.01126329],"genre_scores_gemma":[0.2143292,0.002400542,0.7475693,0.002431199,0.0004200379,0.007940086,0.004026373,0.001048744,0.01983448],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01595271,"threshold_uncertainty_score":0.08436704,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02200920302136245,"score_gpt":0.2891835237600053,"score_spread":0.2671743207386428,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}