{"id":"W1973393941","doi":"10.1021/jp106754k","title":"Fast Molecular Beacon Hybridization in Organic Solvents with Improved Target Specificity","year":2010,"lang":"en","type":"article","venue":"The Journal of Physical Chemistry B","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":56,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"","keywords":"Molecular beacon; Chemistry; Fluorophore; Solvent; Ethylene glycol; Formamide; Acetonitrile; DNA; Dimethylformamide; Fluorescence; Aqueous solution; Dimethyl sulfoxide; Methanol; Ethanol; Photochemistry; Combinatorial chemistry; Organic chemistry; Oligonucleotide; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001313201,0.001153603,0.000723919,0.0006943455,0.0002915804,0.0006472458,0.0006719387,0.0007704646,0.001180273],"category_scores_gemma":[0.001841962,0.0006136722,0.0004233707,0.0006947579,0.0006122114,0.0008695471,0.0007556342,0.001043021,0.001326567],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004381143,"about_ca_system_score_gemma":0.0003825285,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004330338,"about_ca_topic_score_gemma":0.0006154123,"domain_scores_codex":[0.9988839,0.0003815734,0.00006578868,0.0002570617,0.0002865669,0.0001251298],"domain_scores_gemma":[0.9990232,0.0004853143,0.0002067733,0.0000592268,0.0001673074,0.0000581344],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005541405,0.00001137776,0.00005076847,0.0001325491,0.000004804391,0.00003514299,0.00003886152,0.0001631324,0.9955127,0.0002447944,0.00005317264,0.003697422],"study_design_scores_gemma":[0.00001096792,0.0001503964,0.0001147653,0.00001351245,0.00001130097,0.0001128886,0.00001521358,0.0007070954,0.9960344,0.0001002308,0.002719912,0.000009354659],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.524278,0.02003403,0.4467163,0.0007631662,0.0004224743,0.0002959078,0.0003814465,0.002467779,0.004640893],"genre_scores_gemma":[0.6395279,0.01518419,0.3320194,0.0006966045,0.0001870616,0.0007076865,0.0008877407,0.0004099241,0.01037942],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001313201,"threshold_uncertainty_score":0.006944895,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002937664034420914,"score_gpt":0.2245281521968769,"score_spread":0.221590488162456,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}