{"id":"W1973432469","doi":"10.1016/s0020-7519(00)00019-9","title":"Overcoming codon bias: A method for high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli","year":2000,"lang":"en","type":"article","venue":"International Journal for Parasitology","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":142,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Petroleum Technology Research Centre","keywords":"Biology; Gene; Codon usage bias; Escherichia coli; Plasmid; Parasite hosting; Genetics; Start codon; Recombinant DNA; Messenger RNA; Genome","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003682815,0.0001353176,0.000227216,0.00008139383,0.00006900317,0.00001953932,0.0002140021,0.0001912829,0.0001267136],"category_scores_gemma":[0.00008848789,0.0001223168,0.00008910368,0.00002547505,0.0000344905,0.00000860335,0.00004016245,0.00006788453,0.000001551726],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003883466,"about_ca_system_score_gemma":0.00005723674,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002108377,"about_ca_topic_score_gemma":0.0004874137,"domain_scores_codex":[0.9989942,0.0001086925,0.0003522479,0.000232517,0.0001223605,0.0001900352],"domain_scores_gemma":[0.9993764,0.0001541497,0.0002121422,0.00009600355,0.0001097302,0.00005163982],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002551995,0.0000673305,0.0178104,0.00001219401,0.000138059,0.0000030607,0.00009582184,0.0002287158,0.9694763,0.0004645794,0.0003932595,0.008758225],"study_design_scores_gemma":[0.002232587,0.0004197058,0.009170043,0.00006368295,0.00002176296,0.0001923595,0.00002194244,0.0002883721,0.9448782,0.002778955,0.03976163,0.0001707697],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9743691,0.0002922997,0.02428918,0.0001801518,0.0003771925,0.0002528124,0.0001225638,0.000002630354,0.0001140406],"genre_scores_gemma":[0.9353439,0.0001511158,0.06241463,0.0003127344,0.0002854368,0.00008329418,0.0000330247,0.000026206,0.001349652],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03936837,"threshold_uncertainty_score":0.4987934,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03670323543396833,"score_gpt":0.3506382599803504,"score_spread":0.313935024546382,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}