{"id":"W1974449499","doi":"10.2144/000113354","title":"A Proximity Ligation Assay Using Transiently Transfected, Epitope-Tagged Proteins: Application for in Situ Detection of Dimerized Receptor Tyrosine Kinases","year":2010,"lang":"en","type":"article","venue":"BioTechniques","topic":"HER2/EGFR in Cancer Research","field":"Medicine","cited_by":46,"is_retracted":false,"has_abstract":true,"ca_institutions":"SickKids Foundation; Toronto Western Hospital; Hospital for Sick Children","funders":"National Cancer Institute; Sick Kids Foundation","keywords":"Proximity ligation assay; Epitope; Epidermal growth factor receptor; Immunoprecipitation; Receptor tyrosine kinase; Tyrosine kinase; Receptor; Transfection; Molecular biology; Biology; Cell biology; Target protein; Ligand (biochemistry); Chemistry; Computational biology; Antibody; Biochemistry; Immunology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001107009,0.001087422,0.0009555557,0.0006653502,0.0006287013,0.0008410961,0.0007031424,0.001115197,0.001635541],"category_scores_gemma":[0.0007315296,0.0005368323,0.0006472273,0.0005586871,0.0006062326,0.0008503664,0.0007425885,0.002075454,0.001130852],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004582572,"about_ca_system_score_gemma":0.0003456034,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002595076,"about_ca_topic_score_gemma":0.0003649087,"domain_scores_codex":[0.9986593,0.0004967158,0.00008817616,0.0003807211,0.0002860692,0.00008899741],"domain_scores_gemma":[0.9992031,0.0003936175,0.0001528635,0.00009746241,0.00007880892,0.00007415722],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003342959,0.00002498169,0.00003855623,0.00005220076,0.000006493687,0.00002750191,0.00002468384,0.00003637596,0.9985164,0.0001124118,0.00003818128,0.001088717],"study_design_scores_gemma":[0.000008441256,0.0001636592,0.0003859091,0.000004385769,0.00001965093,0.000299092,0.00001804399,0.001991121,0.9954113,0.00006731911,0.001622375,0.000008693327],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2638125,0.003071328,0.7275934,0.0005502414,0.0001693419,0.0003326433,0.0003914537,0.001311039,0.00276807],"genre_scores_gemma":[0.5356902,0.004431673,0.4494043,0.0003023353,0.0001223261,0.00082681,0.0009902252,0.000147582,0.008084523],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001635541,"threshold_uncertainty_score":0.005854487,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03177015599344328,"score_gpt":0.3461302312628107,"score_spread":0.3143600752693674,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}