{"id":"W1974449499","doi":"10.2144/000113354","title":"A Proximity Ligation Assay Using Transiently Transfected, Epitope-Tagged Proteins: Application for in Situ Detection of Dimerized Receptor Tyrosine Kinases","year":2010,"lang":"en","type":"article","venue":"BioTechniques","topic":"HER2/EGFR in Cancer Research","field":"Medicine","cited_by":46,"is_retracted":false,"has_abstract":true,"ca_institutions":"SickKids Foundation; Toronto Western Hospital; Hospital for Sick Children","funders":"National Cancer Institute; Sick Kids Foundation","keywords":"Proximity ligation assay; Epitope; Epidermal growth factor receptor; Immunoprecipitation; Receptor tyrosine kinase; Tyrosine kinase; Receptor; Transfection; Molecular biology; Biology; Cell biology; Target protein; Ligand (biochemistry); Chemistry; Computational biology; Antibody; Biochemistry; Immunology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001081279,0.000161722,0.0003400208,0.0002987492,0.00006136418,0.00001428748,0.0001062029,0.0003826645,0.00001294356],"category_scores_gemma":[0.0003722369,0.0001525335,0.0001064,0.0005552638,0.0001293216,0.0001515999,0.00001070661,0.0004343935,0.00000111705],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001727635,"about_ca_system_score_gemma":0.0002061921,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008289839,"about_ca_topic_score_gemma":0.0006237468,"domain_scores_codex":[0.9985095,0.000115024,0.0004487101,0.0003604562,0.000315131,0.0002512131],"domain_scores_gemma":[0.9991002,0.00007550632,0.0001264727,0.0003350475,0.0002834334,0.00007931692],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000750248,0.0002493268,0.0002585842,0.0004713818,0.0000107971,5.089956e-7,0.0001474285,3.976534e-7,0.9109133,0.00005696521,0.000009025083,0.08713196],"study_design_scores_gemma":[0.001145408,0.000659132,0.001447469,0.0003240223,0.00003387492,0.000006210313,0.00001677813,0.0008792529,0.9929861,0.0001733864,0.002187679,0.0001406208],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7492151,0.00008308542,0.2434125,0.0005687571,0.00006497392,0.006387278,0.00002714693,0.0002020833,0.00003913087],"genre_scores_gemma":[0.9509553,0.00007924889,0.04704898,0.00002613893,0.0001334158,0.001655714,0.00004928389,0.00004002064,0.00001190169],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2017402,"threshold_uncertainty_score":0.6220137,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03177015599344328,"score_gpt":0.3461302312628107,"score_spread":0.3143600752693674,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}