{"id":"W1974465538","doi":"10.1021/bc034130j","title":"Efficient and Expedient Two-Step Pyranose-Retaining Fluorescein Conjugation of Complex Reducing Oligosaccharides:  Galectin Oligosaccharide Specificity Studies in a Fluorescence Polarization Assay","year":2003,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Galectins and Cancer Biology","field":"Immunology and Microbiology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"","keywords":"Chemistry; Galectin; Pyranose; Fluorescence anisotropy; Fluorescence; Oligosaccharide; Conjugate; Acylation; Fluorescein; Fluorescent labelling; Biochemistry; Chromatography; Combinatorial chemistry; Biophysics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004462428,0.0005438764,0.0004968965,0.0001715748,0.000136193,0.0003756439,0.0004149819,0.0004832347,0.0005245894],"category_scores_gemma":[0.0004594101,0.0002103471,0.0002520735,0.0002789555,0.0002688791,0.000334899,0.0003842393,0.0007345579,0.0002552134],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003779379,"about_ca_system_score_gemma":0.0002721697,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005317446,"about_ca_topic_score_gemma":0.0007181222,"domain_scores_codex":[0.9996296,0.00009599453,0.00002038492,0.00006743066,0.0001208812,0.00006574512],"domain_scores_gemma":[0.999755,0.00007796054,0.0000553341,0.0000321369,0.0000503043,0.00002938503],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002558251,0.00001694266,0.00004527183,0.00001605605,0.000002927509,0.00001091374,0.000007461389,0.00006764261,0.9983717,0.00005618266,0.00001281409,0.001366458],"study_design_scores_gemma":[0.000003659295,0.00003843027,0.0002604552,8.068072e-7,0.000002824207,0.00005193054,0.000002628735,0.0004458308,0.9987828,0.00001505643,0.0003923127,0.000003142466],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8900902,0.0007702599,0.1071242,0.000129585,0.00002597449,0.0002516076,0.0003268721,0.0002060122,0.001075279],"genre_scores_gemma":[0.9039853,0.001129373,0.09131441,0.00009169694,0.000009755369,0.0002514326,0.0006053427,0.00008158868,0.00253116],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005438764,"threshold_uncertainty_score":0.002742171,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03914638673926064,"score_gpt":0.2935927127742994,"score_spread":0.2544463260350388,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}