{"id":"W1974716325","doi":"10.1016/j.ab.2009.05.023","title":"Obtaining highly purified intrinsically disordered protein by boiling lysis and single step ion exchange","year":2009,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Protein Structure and Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":63,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; University of California, Riverside","keywords":"Chemistry; Lysis; Proteases; Chromatography; Escherichia coli; Ion chromatography; Protease; Boiling; Protein purification; Proteinase K; Sonication; Biochemistry; Enzyme; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001282234,0.0002646478,0.000251771,0.0000317345,0.0001050904,0.00007346443,0.0001976258,0.0003652102,0.00001414507],"category_scores_gemma":[0.000231567,0.0002527396,0.0001058544,0.0001553028,0.0001318064,0.000006834986,0.0001162112,0.0001859657,0.000002667131],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002669467,"about_ca_system_score_gemma":0.00004336259,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009254705,"about_ca_topic_score_gemma":0.000003201275,"domain_scores_codex":[0.9985034,0.00003027269,0.0002860374,0.0006045543,0.0001921612,0.0003836155],"domain_scores_gemma":[0.9992108,0.00001319536,0.00009012547,0.0003987837,0.00007786287,0.0002091839],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009931723,0.00008882708,0.0001169226,0.00003617592,0.00004831125,0.000006307314,0.0000107306,0.000001848884,0.9811906,0.0001183073,0.0007790365,0.01750357],"study_design_scores_gemma":[0.0006863432,0.0003897565,0.000132618,0.00003696149,0.00006076273,0.00001550742,0.00005405236,0.0008000586,0.9912016,0.000485864,0.005704269,0.0004322312],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9913534,0.001648739,0.003630845,0.0009346319,0.00002236545,0.0001820985,0.00002909918,0.00003848358,0.002160325],"genre_scores_gemma":[0.9961939,0.00008915742,0.00161273,0.0004151259,0.0002438443,0.00001220576,0.0003257722,0.00001906547,0.001088184],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01707134,"threshold_uncertainty_score":0.9999925,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005013212844171837,"score_gpt":0.2196361639149429,"score_spread":0.2146229510707711,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}