{"id":"W1975071492","doi":"10.1385/mb:31:3:187","title":"A Novel In Vitro Transcription Method for Producing siRNAs Without Specific Sequence Requirements","year":2005,"lang":"en","type":"article","venue":"Molecular Biotechnology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute","funders":"","keywords":"Small interfering RNA; Trans-acting siRNA; RNA interference; Biology; Transcription (linguistics); RNA silencing; Gene silencing; RNA; RNase H; RNase P; Oligonucleotide; Molecular biology; DNA; Cell biology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005787528,0.001275202,0.0009379136,0.0006807703,0.0006073369,0.0006576843,0.0007306199,0.0007334519,0.001294923],"category_scores_gemma":[0.0004050294,0.0007246963,0.0009489458,0.0003883292,0.000459344,0.0004997386,0.0005767071,0.002443344,0.001741279],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006313331,"about_ca_system_score_gemma":0.0006990887,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005595613,"about_ca_topic_score_gemma":0.00155485,"domain_scores_codex":[0.9994024,0.0001015497,0.00006592982,0.0001335742,0.0002355269,0.00006099769],"domain_scores_gemma":[0.9996191,0.0001133371,0.00008468612,0.00006585997,0.00006698719,0.00004994528],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002161415,0.00001464186,0.00001782556,0.00005395697,0.000003791814,0.00003454998,0.00001482969,0.00003565576,0.9970083,0.00017722,0.000116357,0.002501264],"study_design_scores_gemma":[0.000007562609,0.00005281343,0.0001374689,0.000004329806,0.00002052813,0.0002340632,0.000004730128,0.000457325,0.9923744,0.00007694068,0.006617263,0.00001252593],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1083991,0.003876695,0.8757707,0.0005081433,0.0009185207,0.0006128186,0.001257847,0.003049484,0.00560677],"genre_scores_gemma":[0.316814,0.005645077,0.648743,0.0005195487,0.000371413,0.001120297,0.005369634,0.0009128107,0.02050428],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001294923,"threshold_uncertainty_score":0.004580677,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03398083903826116,"score_gpt":0.3077831833486565,"score_spread":0.2738023443103953,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}