{"id":"W1975550351","doi":"10.1111/j.1574-6968.2001.tb10685.x","title":"PhaR, a protein of unknown function conserved among short-chain-length polyhydroxyalkanoic acids producing bacteria, is a DNA-binding protein and represses<i>Paracoccus denitrificans phaP</i>expression in vitro","year":2001,"lang":"en","type":"article","venue":"FEMS Microbiology Letters","topic":"biodegradable polymer synthesis and properties","field":"Materials Science","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"RIKEN; Federation of European Microbiological Societies; Public Health Agency of Canada","keywords":"Biology; Biochemistry; Escherichia coli; Electrophoretic mobility shift assay; Molecular biology; Recombinant DNA; Molecular mass; Binding protein; Gel electrophoresis; FLAG-tag; Affinity chromatography; Nucleic acid sequence; Gene; Gene expression; Fusion protein; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0008085768,0.0004162937,0.0006586965,0.0002422586,0.000241528,0.00008855768,0.0003398324,0.0002748384,0.0002029906],"category_scores_gemma":[0.00008781416,0.0003448229,0.00009684169,0.0002840684,0.0005876754,0.0003486971,0.0001587845,0.0002999861,0.00001448263],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006788644,"about_ca_system_score_gemma":0.00006139686,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007311863,"about_ca_topic_score_gemma":0.00009678936,"domain_scores_codex":[0.9968627,0.0005179713,0.0007678936,0.001014624,0.0001190203,0.0007178098],"domain_scores_gemma":[0.9988632,0.00007605978,0.0003526963,0.0005560938,0.00005216055,0.00009980476],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001188797,0.0001062692,0.0042806,0.00009850628,0.0000324013,0.0000231236,0.0007190426,8.784983e-7,0.9917629,0.000001353485,0.0001816611,0.001604433],"study_design_scores_gemma":[0.0007125355,0.0001177602,0.001006662,0.0004455895,0.00003710695,0.00005342671,0.0001041794,0.00002549692,0.9962515,0.000004855899,0.000827494,0.0004134301],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.995601,0.001574077,0.0001039524,0.00108471,0.0002848892,0.001139047,0.00009242309,0.00008960273,0.00003027245],"genre_scores_gemma":[0.9978745,0.00002670264,0.000908297,0.0003383566,0.00009196989,0.0002931157,0.00004019661,0.00004164489,0.0003852191],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004488529,"threshold_uncertainty_score":0.9999004,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.015911565517717,"score_gpt":0.2080464472961552,"score_spread":0.1921348817784382,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}