{"id":"W1975550351","doi":"10.1111/j.1574-6968.2001.tb10685.x","title":"PhaR, a protein of unknown function conserved among short-chain-length polyhydroxyalkanoic acids producing bacteria, is a DNA-binding protein and represses<i>Paracoccus denitrificans phaP</i>expression in vitro","year":2001,"lang":"en","type":"article","venue":"FEMS Microbiology Letters","topic":"biodegradable polymer synthesis and properties","field":"Materials Science","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"RIKEN; Federation of European Microbiological Societies; Public Health Agency of Canada","keywords":"Biology; Biochemistry; Escherichia coli; Electrophoretic mobility shift assay; Molecular biology; Recombinant DNA; Molecular mass; Binding protein; Gel electrophoresis; FLAG-tag; Affinity chromatography; Nucleic acid sequence; Gene; Gene expression; Fusion protein; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001404934,0.0005827309,0.0003153169,0.0002109822,0.0001870132,0.0003171657,0.0001860167,0.0003488628,0.0008345088],"category_scores_gemma":[0.0002426683,0.0001669072,0.0002503657,0.0001456933,0.0003408293,0.0002450737,0.0001632723,0.0003527968,0.0009010896],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003170835,"about_ca_system_score_gemma":0.0002297202,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005283272,"about_ca_topic_score_gemma":0.0004501812,"domain_scores_codex":[0.9998684,0.0000228564,0.00000663591,0.00004084281,0.00003693794,0.0000242323],"domain_scores_gemma":[0.9998024,0.00005473393,0.00005927,0.00002292829,0.0000252738,0.00003539899],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003220488,0.00001477202,0.0002302921,0.00002693881,0.000001899509,0.00007619133,0.000006827542,0.00002322915,0.9983522,0.00006865212,0.00001950245,0.001147376],"study_design_scores_gemma":[0.00001338449,0.0001969517,0.0109292,0.00000818233,0.00001409626,0.001514538,0.00005944878,0.001301833,0.982239,0.0001277346,0.003587855,0.000007789419],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9818916,0.003398169,0.01195441,0.0002185896,0.00002621001,0.00004210499,0.0006010387,0.000227041,0.00164085],"genre_scores_gemma":[0.9768291,0.002112899,0.01280956,0.0001008773,0.00003093337,0.00003704352,0.003079576,0.00007590485,0.004924055],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008345088,"threshold_uncertainty_score":0.002791643,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.015911565517717,"score_gpt":0.2080464472961552,"score_spread":0.1921348817784382,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}