{"id":"W1976673761","doi":"10.1007/s003350010238","title":"Transgenic mice for the preparation of puromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selection","year":2001,"lang":"en","type":"article","venue":"Mammalian Genome","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":false,"ca_institutions":"Children's & Women's Health Centre of British Columbia; University of British Columbia","funders":"National Institute of Mental Health","keywords":"Embryonic stem cell; Biology; Puromycin; Transgene; Cell biology; Selection (genetic algorithm); Embryo; Genetically modified mouse; Fibroblast; Stem cell; Genetics; Cell culture; Gene; Protein biosynthesis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00140074,0.002213683,0.001395909,0.003928434,0.001550988,0.001204192,0.002899134,0.003273474,0.04001285],"category_scores_gemma":[0.0005699294,0.001823057,0.001549035,0.00140718,0.0009370722,0.001188008,0.001000675,0.005228631,0.01768157],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001143945,"about_ca_system_score_gemma":0.00103721,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001208338,"about_ca_topic_score_gemma":0.005796893,"domain_scores_codex":[0.9987648,0.0001328311,0.0001422887,0.0003445452,0.0003906486,0.0002249143],"domain_scores_gemma":[0.9985423,0.0002775517,0.0003714761,0.0002431404,0.0001571326,0.0004083278],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003260072,0.0002909152,0.000212054,0.0001865161,0.0000506103,0.0002377793,0.0001068765,0.0001037588,0.9835432,0.00355963,0.004373831,0.007008857],"study_design_scores_gemma":[0.0003713258,0.000440745,0.004171346,0.0002336887,0.0002289206,0.002411325,0.00009133716,0.0014022,0.8679903,0.001575319,0.1209909,0.00009248294],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3141211,0.009266648,0.4651617,0.004496835,0.002739983,0.006516785,0.1134394,0.02723034,0.0570272],"genre_scores_gemma":[0.2384185,0.01166171,0.3475451,0.001935358,0.0003495216,0.00698111,0.09019262,0.007694888,0.2952212],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.04001285,"threshold_uncertainty_score":0.1338563,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009229808227061901,"score_gpt":0.2609108533244894,"score_spread":0.2516810450974276,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}