{"id":"W1976696370","doi":"10.1128/aem.68.12.6152-6161.2002","title":"Novel Food-Grade Plasmid Vector Based on Melibiose Fermentation for the Genetic Engineering of <i>Lactococcus lactis</i>","year":2002,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Probiotics and Fermented Foods","field":"Agricultural and Biological Sciences","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada; Université Laval","funders":"Natural Sciences and Engineering Research Council of Canada; Dairy Farmers of Canada","keywords":"Melibiose; Lactococcus lactis; Biology; Plasmid; Pediococcus acidilactici; Shuttle vector; Selectable marker; Gene; Lactococcus; Fermentation; Expression vector; Biochemistry; Genetics; Recombinant DNA; Bacteria; Vector (molecular biology); Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00003822918,0.0001243065,0.0001178515,0.000008285473,0.00009491111,0.000009268175,0.00009848074,0.00008243191,0.00009167482],"category_scores_gemma":[0.000001984399,0.00005148716,0.00004713467,0.00003835155,0.0000806045,0.00001177917,0.00003144371,0.00006481875,0.000004877959],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001838404,"about_ca_system_score_gemma":6.812489e-7,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003816065,"about_ca_topic_score_gemma":0.000003315049,"domain_scores_codex":[0.9994434,0.000006962056,0.0001392246,0.0002017216,0.00003034831,0.0001784129],"domain_scores_gemma":[0.9997103,0.0001479492,0.00006023258,0.00004739268,0.000001358931,0.00003272726],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002887773,0.0001416178,0.0001633783,0.0000055464,0.0000184381,8.018459e-8,0.00001876185,0.0002016271,0.9947373,0.00009838892,0.00006151626,0.004524444],"study_design_scores_gemma":[0.002552738,0.002906508,0.121474,0.00002371219,0.000100935,0.00001993554,0.0002652455,0.01128322,0.8371611,0.00005595449,0.02356532,0.0005913162],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981187,0.0001467269,0.0001651521,0.0004145261,0.00008693699,0.0005296707,0.0004663187,0.00001398494,0.00005797805],"genre_scores_gemma":[0.9989542,0.00006916584,0.0003308905,0.0004127011,0.00004615288,0.00004900504,0.0001244504,0.000002066953,0.00001133114],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1575762,"threshold_uncertainty_score":0.2099585,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01180416732303779,"score_gpt":0.1585391049123752,"score_spread":0.1467349375893374,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}