{"id":"W1976696370","doi":"10.1128/aem.68.12.6152-6161.2002","title":"Novel Food-Grade Plasmid Vector Based on Melibiose Fermentation for the Genetic Engineering of <i>Lactococcus lactis</i>","year":2002,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Probiotics and Fermented Foods","field":"Agricultural and Biological Sciences","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada; Université Laval","funders":"Natural Sciences and Engineering Research Council of Canada; Dairy Farmers of Canada","keywords":"Melibiose; Lactococcus lactis; Biology; Plasmid; Pediococcus acidilactici; Shuttle vector; Selectable marker; Gene; Lactococcus; Fermentation; Expression vector; Biochemistry; Genetics; Recombinant DNA; Bacteria; Vector (molecular biology); Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002169551,0.0007312465,0.0004573983,0.0003891174,0.0001875728,0.0003621287,0.0005237083,0.000396322,0.00109042],"category_scores_gemma":[0.0002372805,0.0002287159,0.0004064677,0.0005194893,0.0001811626,0.0003670298,0.0004186109,0.00105541,0.001027756],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003598402,"about_ca_system_score_gemma":0.0003071978,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004527444,"about_ca_topic_score_gemma":0.0005359366,"domain_scores_codex":[0.9998117,0.00003093363,0.00002325792,0.000043425,0.00005591926,0.00003479946],"domain_scores_gemma":[0.9998766,0.00002655386,0.00004121679,0.00001661443,0.00001413804,0.00002474471],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006023433,0.00004990141,0.0001375766,0.00004785512,0.000005164165,0.00005478131,0.00001692188,0.00005892711,0.9948947,0.0002641993,0.00008558283,0.004324133],"study_design_scores_gemma":[0.00005317877,0.0004563223,0.003055657,0.00001998535,0.0000437508,0.001076079,0.00003033138,0.001660668,0.9763387,0.0001892149,0.01705617,0.0000199562],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8340106,0.002031234,0.1526325,0.0003860705,0.0002277833,0.0006844265,0.003974785,0.001714685,0.004337856],"genre_scores_gemma":[0.8151821,0.001835452,0.1478478,0.0001811619,0.00005125828,0.0005829425,0.01812731,0.0004158977,0.01577612],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00109042,"threshold_uncertainty_score":0.003647804,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01180416732303779,"score_gpt":0.1585391049123752,"score_spread":0.1467349375893374,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}