{"id":"W1976708245","doi":"10.1016/j.gene.2004.10.016","title":"Drag&Drop cloning in yeast","year":2004,"lang":"en","type":"article","venue":"Gene","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":181,"is_retracted":false,"has_abstract":false,"ca_institutions":"Royal Victoria Hospital; Biotechnology Research Institute; McGill University","funders":"Natural Sciences and Engineering Research Council of Canada; Deutsche Forschungsgemeinschaft","keywords":"Biology; Multiple cloning site; Selectable marker; Cloning (programming); Genetics; Saccharomyces cerevisiae; Gene; Computational biology; Homologous recombination; Expression cloning; Cloning vector; Expression vector; Molecular cloning; Shuttle vector; Vector (molecular biology); Plasmid; Complementary DNA; Recombinant DNA; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004996379,0.001600316,0.001693807,0.001794575,0.001420332,0.001846135,0.00344633,0.001254455,0.01975752],"category_scores_gemma":[0.0007996815,0.001511927,0.001253183,0.001349633,0.0009768631,0.0009704331,0.001639704,0.004021195,0.03334235],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001003252,"about_ca_system_score_gemma":0.0007549166,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001929542,"about_ca_topic_score_gemma":0.003638382,"domain_scores_codex":[0.9993538,0.00004974505,0.00006678292,0.000148894,0.0002978918,0.00008271811],"domain_scores_gemma":[0.9994677,0.0001754018,0.0000616465,0.0001184949,0.0000532941,0.0001236388],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001147899,0.0003180372,0.0006529428,0.00141319,0.00007795196,0.0008574323,0.0003060026,0.000603109,0.8752549,0.01060267,0.04163142,0.06713439],"study_design_scores_gemma":[0.0002022837,0.00008800333,0.0009447464,0.00006646795,0.00006357579,0.0006704803,0.00004878129,0.002119295,0.8391863,0.0009901295,0.1555665,0.00005346914],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1150459,0.003837751,0.6262088,0.00284166,0.002444453,0.002996633,0.09688497,0.09983942,0.04990042],"genre_scores_gemma":[0.2455692,0.005410841,0.4448779,0.001538818,0.0004259099,0.002918845,0.1128352,0.03162694,0.1547964],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.01975752,"threshold_uncertainty_score":0.06609547,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01679888823992265,"score_gpt":0.2828135824325266,"score_spread":0.266014694192604,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}