{"id":"W1976737414","doi":"10.3791/52522","title":"Assessing Transmissible Spongiform Encephalopathy Species Barriers with an <em>In Vitro</em> Prion Protein Conversion Assay","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Prion Diseases and Protein Misfolding","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"U.S. Geological Survey; National Institutes of Health; Michigan Department of Natural Resources; Wisconsin Department of Natural Resources","keywords":"In vitro; Chronic wasting disease; Prion protein; Transmissible spongiform encephalopathy; In vivo; Bovine spongiform encephalopathy; Odocoileus; Bioassay; In vitro toxicology; Biology; Rodent; Virology; Proteinase K; Scrapie; Chemistry; Biochemistry; Disease; Enzyme; Medicine; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001104844,0.001106063,0.0004937177,0.0007410188,0.0003582836,0.0005407275,0.0005113101,0.0006336521,0.001368631],"category_scores_gemma":[0.0007570235,0.0004070171,0.0004373525,0.0003905629,0.0004492156,0.0004567502,0.0005355747,0.001304735,0.0006664111],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002410656,"about_ca_system_score_gemma":0.0002073403,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000780234,"about_ca_topic_score_gemma":0.001386778,"domain_scores_codex":[0.9988417,0.000367756,0.0001609519,0.0001972287,0.0003344215,0.00009787246],"domain_scores_gemma":[0.9985756,0.0004727501,0.0004113897,0.0001674693,0.0002623106,0.0001103988],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005564759,0.00006612185,0.000644734,0.00004042911,0.00001226035,0.00004295042,0.00004180252,0.00006521367,0.997745,0.00008395854,0.00002901793,0.001172849],"study_design_scores_gemma":[0.000005626822,0.0006659381,0.00653497,0.00001006105,0.00003804563,0.0002932279,0.00003954652,0.000759421,0.9903133,0.00006273615,0.001263479,0.00001366876],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8805989,0.002429712,0.1081791,0.00009660128,0.0000859901,0.0003569646,0.0006709933,0.000573313,0.007008353],"genre_scores_gemma":[0.8764386,0.003293046,0.1090588,0.000195507,0.00004609705,0.0006385393,0.002178578,0.0001534559,0.007997353],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001368631,"threshold_uncertainty_score":0.005843043,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02509932715087696,"score_gpt":0.3489061840969577,"score_spread":0.3238068569460807,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}