{"id":"W1976791458","doi":"10.1016/j.jmb.2003.12.071","title":"High-resolution Crystal Structure of Arthrobacter aurescens Chondroitin AC Lyase: An Enzyme–Substrate Complex Defines the Catalytic Mechanism","year":2004,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"Proteoglycans and glycosaminoglycans research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":113,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute","funders":"National Institute of General Medical Sciences; National Heart, Lung, and Blood Institute; National Institutes of Health","keywords":"Tetrasaccharide; Lyase; Chondroitin; Chemistry; Chondroitin sulfate; Glucuronic acid; Stereochemistry; Substrate (aquarium); Dermatan sulfate; Active site; Enzyme; Biochemistry; Glycosaminoglycan; Polysaccharide; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003202079,0.0005016704,0.0006177942,0.0002083951,0.0005030209,0.001209452,0.0008034671,0.0007982966,0.0007822001],"category_scores_gemma":[0.0005018605,0.0003571604,0.0002570598,0.0004251492,0.0002956926,0.000406403,0.0002195013,0.001746746,0.0005579745],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001534196,"about_ca_system_score_gemma":0.001109971,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.008970232,"about_ca_topic_score_gemma":0.01090638,"domain_scores_codex":[0.9998878,0.00001285853,0.00001204462,0.00002431055,0.00004412445,0.00001886392],"domain_scores_gemma":[0.9996837,0.00005313494,0.00005563615,0.00002827514,0.00008238039,0.00009681875],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001139961,0.0003959888,0.003850474,0.0002516342,0.00007375706,0.001131088,0.0004730316,0.002714656,0.9777498,0.001789422,0.003571352,0.00685883],"study_design_scores_gemma":[0.0006706005,0.0005677239,0.0608899,0.0001189915,0.0002920873,0.003756825,0.001265489,0.04272307,0.8435729,0.002052126,0.04387903,0.0002111587],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9873697,0.003662234,0.003520095,0.0007628491,0.0001009962,0.00003491312,0.001872508,0.0002500792,0.002426687],"genre_scores_gemma":[0.9759113,0.002654854,0.007959411,0.000278942,0.00003417025,0.00002913737,0.009342872,0.0001011359,0.003688097],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008970232,"threshold_uncertainty_score":0.01783603,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01327777284886783,"score_gpt":0.2726679459495885,"score_spread":0.2593901731007207,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}