{"id":"W1977436561","doi":"10.3791/51395","title":"Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy","year":2014,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Analytical Chemistry and Sensors","field":"Chemical Engineering","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke","funders":"Canadian Institutes of Health Research","keywords":"Cytoplasm; Organelle; Endosome; Endocytic cycle; Confocal microscopy; Chemistry; Cellular compartment; Intracellular; Cell biology; Cytosol; Biophysics; Fluorescence microscope; Confocal; Vesicle; Live cell imaging; Fluorescence; Cell; Biochemistry; Endocytosis; Biology; Membrane; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009454412,0.0009072981,0.000882643,0.0009888097,0.000481223,0.0007495636,0.001132654,0.001172699,0.001817946],"category_scores_gemma":[0.0006846256,0.000407802,0.0006003759,0.0006992365,0.0005695918,0.0009970712,0.001421935,0.001465537,0.0009957806],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009239831,"about_ca_system_score_gemma":0.0005770013,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008909684,"about_ca_topic_score_gemma":0.001097211,"domain_scores_codex":[0.9988978,0.0001805692,0.00006596419,0.0004000394,0.0003150074,0.0001406867],"domain_scores_gemma":[0.9995127,0.0001304983,0.00006891485,0.00008209167,0.0001572848,0.00004860496],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003808772,0.00001025363,0.0000804252,0.00009081666,0.000007102743,0.00002612568,0.00002169062,0.00009073226,0.9972568,0.0002100071,0.00008100065,0.002086987],"study_design_scores_gemma":[0.00001289471,0.00007213357,0.001332444,0.00002048519,0.00001978591,0.0002670627,0.00002754554,0.003888189,0.9914085,0.0001775831,0.002749442,0.00002395482],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3480676,0.005751566,0.6332405,0.0004022933,0.0001740007,0.0005917523,0.001945006,0.002561322,0.007265889],"genre_scores_gemma":[0.3489701,0.006165413,0.6380647,0.0003024774,0.00008096827,0.00143027,0.001196935,0.000414086,0.003375075],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001817946,"threshold_uncertainty_score":0.006703973,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03877677112214657,"score_gpt":0.3871374622659358,"score_spread":0.3483606911437892,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}