{"id":"W1977666161","doi":"10.1007/s10646-009-0347-9","title":"Development of a direct DNA extraction protocol for real-time PCR detection of Giardia lamblia from surface water","year":2009,"lang":"en","type":"article","venue":"Ecotoxicology","topic":"Parasitic Infections and Diagnostics","field":"Immunology and Microbiology","cited_by":25,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"DNA extraction; Giardia lamblia; Chromatography; Giardia; Extraction (chemistry); Immunomagnetic separation; Biology; Incubation period; Polymerase chain reaction; Incubation; Proteinase K; DNA; Molecular biology; Chemistry; Microbiology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001750532,0.001382555,0.001339924,0.001560146,0.0007254636,0.0007956836,0.001527863,0.001173089,0.002546748],"category_scores_gemma":[0.002665481,0.001422849,0.001055181,0.0007106541,0.0007449154,0.0009050813,0.0008359482,0.002563187,0.003382454],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004689304,"about_ca_system_score_gemma":0.001886154,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001826785,"about_ca_topic_score_gemma":0.005264689,"domain_scores_codex":[0.9978476,0.0003474348,0.0002769904,0.0004911413,0.0008815808,0.0001553245],"domain_scores_gemma":[0.9980829,0.0005960151,0.0001763584,0.0002694456,0.0007630605,0.0001122701],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002440731,0.00005845308,0.000344692,0.0001304117,0.00000812406,0.00004197597,0.00004272622,0.0000907739,0.9916728,0.0001023452,0.0001209291,0.007362369],"study_design_scores_gemma":[0.00003712386,0.0006553685,0.003828565,0.00008400473,0.00008390233,0.0003522644,0.00005975752,0.002011448,0.9818649,0.0002691347,0.01071255,0.0000409511],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1194447,0.001325255,0.8657175,0.0003635249,0.0003950825,0.005477179,0.002503499,0.001469174,0.00330412],"genre_scores_gemma":[0.06620278,0.001661164,0.9129061,0.0003652378,0.00008645807,0.0050037,0.007293565,0.000275857,0.006205096],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002546748,"threshold_uncertainty_score":0.009257793,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01881504251483646,"score_gpt":0.3020029588875809,"score_spread":0.2831879163727445,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}