{"id":"W1978302238","doi":"10.1007/s12033-010-9320-3","title":"Enhancing Functional Expression of Heterologous Burkholderia Lipase in Escherichia coli","year":2010,"lang":"en","type":"article","venue":"Molecular Biotechnology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Periplasmic space; Escherichia coli; Biology; Fusion protein; Chaperone (clinical); Signal peptide; Heterologous expression; Biochemistry; Cytoplasm; GroEL; Lipase; Burkholderia; DsbA; Extracellular; GroES; Molecular biology; Microbiology; Bacteria; Peptide sequence; Recombinant DNA; Gene; Enzyme; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004433065,0.0005162629,0.0003708376,0.0003089224,0.0001781056,0.000713509,0.0003111805,0.000465742,0.0005744499],"category_scores_gemma":[0.0004562609,0.0001754904,0.0003147106,0.0004481994,0.0003009381,0.0003835272,0.000540768,0.0008027224,0.0005982378],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005722466,"about_ca_system_score_gemma":0.0002987777,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001185743,"about_ca_topic_score_gemma":0.001216955,"domain_scores_codex":[0.9996305,0.00008609761,0.00004820563,0.00003448139,0.0001036253,0.0000970161],"domain_scores_gemma":[0.9997447,0.00008703377,0.00004128728,0.00003997027,0.00004606117,0.00004109765],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006716599,0.00004211855,0.000129864,0.00002165823,0.000004289448,0.00004644153,0.00001887055,0.000135032,0.9984844,0.000151356,0.00001446982,0.0008843756],"study_design_scores_gemma":[0.000004707163,0.00003414823,0.0004110421,0.000002483575,0.000005609974,0.00004945611,0.00001702436,0.0005974191,0.9983363,0.00002423577,0.0005149335,0.000002652447],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9861751,0.0005121843,0.0115355,0.0002034568,0.0000402082,0.00004580347,0.0002788693,0.0001154942,0.001093578],"genre_scores_gemma":[0.991546,0.0003725726,0.00510856,0.00005208641,0.000007813413,0.00001859977,0.0004890859,0.00003334408,0.002371969],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001185743,"threshold_uncertainty_score":0.004152,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005113326921996461,"score_gpt":0.2098208235899109,"score_spread":0.2047074966679145,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}