{"id":"W1978302238","doi":"10.1007/s12033-010-9320-3","title":"Enhancing Functional Expression of Heterologous Burkholderia Lipase in Escherichia coli","year":2010,"lang":"en","type":"article","venue":"Molecular Biotechnology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Periplasmic space; Escherichia coli; Biology; Fusion protein; Chaperone (clinical); Signal peptide; Heterologous expression; Biochemistry; Cytoplasm; GroEL; Lipase; Burkholderia; DsbA; Extracellular; GroES; Molecular biology; Microbiology; Bacteria; Peptide sequence; Recombinant DNA; Gene; Enzyme; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","research_integrity"],"consensus_categories":[],"category_scores_codex":[0.0001971645,0.0002442298,0.0002941492,0.0002457313,0.00004432152,0.00001059551,0.0003933756,0.001631217,0.00005814382],"category_scores_gemma":[0.0001667394,0.0002462559,0.000105689,0.0002792744,0.0003808189,0.000003097923,0.0003932494,0.000521782,0.000009113152],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000933257,"about_ca_system_score_gemma":0.00007680355,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003028611,"about_ca_topic_score_gemma":0.0001952895,"domain_scores_codex":[0.9984241,0.00005502256,0.0004152227,0.0005812325,0.0001148481,0.0004095516],"domain_scores_gemma":[0.9989507,0.000008051631,0.0001660313,0.0007482965,0.00006600262,0.00006095821],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000119476,0.0001272319,0.0002796124,0.00001405521,0.00001727069,0.00001941292,0.000006814887,0.00002196331,0.9977471,0.0006824547,0.00005329979,0.0009113118],"study_design_scores_gemma":[0.0007106485,0.0004549159,0.001423943,0.00001177697,0.000009068692,0.00003836132,0.00001985651,0.00001964741,0.9893193,0.0003144636,0.007442485,0.0002354832],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9936165,0.0002283028,0.004592086,0.0006269135,0.0005343352,0.0002326669,0.00001388449,0.00004901734,0.000106243],"genre_scores_gemma":[0.9925296,0.00007375917,0.006915578,0.0002116935,0.00008178025,0.00003899551,0.00007824801,0.00003512466,0.00003522159],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008427746,"threshold_uncertainty_score":0.999999,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005113326921996461,"score_gpt":0.2098208235899109,"score_spread":0.2047074966679145,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}