{"id":"W1978975684","doi":"10.1016/j.bbamem.2008.11.001","title":"Chemical modification of Bacillus thuringiensis Cry1Aa toxin single-cysteine mutants reveals the importance of domain I structural elements in the mechanism of pore formation","year":2008,"lang":"en","type":"article","venue":"Biochimica et Biophysica Acta (BBA) - Biomembranes","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute; Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Fonds Québécois de la Recherche sur la Nature et les Technologies","keywords":"Cysteine; Bacillus thuringiensis; Chemistry; Biochemistry; Mutant; Manduca sexta; Serine; Brush border; Amino acid; Residue (chemistry); Biophysics; Membrane; Vesicle; Biology; Insect; Enzyme; Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009473622,0.0002448077,0.0001420735,0.00008117955,0.0001469512,0.0001681348,0.0002131085,0.0002479611,0.000989591],"category_scores_gemma":[0.0001645602,0.0001082145,0.0001852851,0.0000875393,0.0002383269,0.0001641469,0.0000985657,0.0005649732,0.0002931248],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000315154,"about_ca_system_score_gemma":0.0001343694,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009455888,"about_ca_topic_score_gemma":0.0008804997,"domain_scores_codex":[0.9999402,0.000008935558,0.000005398283,0.00001181902,0.00001790403,0.00001589988],"domain_scores_gemma":[0.9998471,0.00003702338,0.00006235496,0.00001683182,0.00001433247,0.00002239414],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007775477,0.00000971393,0.00009979684,0.0000126778,0.00000276398,0.00003569902,0.00000913667,0.00006036952,0.9992056,0.00006482748,0.00001570488,0.000405961],"study_design_scores_gemma":[0.00000891784,0.00005732431,0.001232926,0.000002188729,0.000006216383,0.0001533554,0.00001039286,0.0003228417,0.9977737,0.00002584332,0.0004033012,0.000002988131],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9970529,0.0002665042,0.001623681,0.00008387102,0.00002105416,0.000009210345,0.0001151069,0.00002758789,0.0008001156],"genre_scores_gemma":[0.9976999,0.000222541,0.0009353079,0.00004585995,0.000004235537,0.000005304094,0.000168272,0.00001912544,0.0008994099],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000989591,"threshold_uncertainty_score":0.003310502,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02049530582366334,"score_gpt":0.2496605474714695,"score_spread":0.2291652416478062,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}