{"id":"W1979089266","doi":"10.1021/bi0344230","title":"Engineering the Primary Substrate Specificity of <i>Streptomyces griseus</i> Trypsin","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; University of British Columbia","funders":"Genome British Columbia; University of Victoria","keywords":"Proteases; Streptomyces griseus; Bacillus subtilis; Trypsin; Mutant; Recombinant DNA; Serine; Protein engineering; Biochemistry; Chemistry; Enzyme; Substrate (aquarium); Streptomycetaceae; Actinomycetales; Stereochemistry; Biology; Streptomyces; Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001703408,0.0003700908,0.0002351473,0.000131741,0.00008594614,0.000298984,0.0002510386,0.0001614628,0.000525984],"category_scores_gemma":[0.0002292425,0.0001332479,0.0001991214,0.0002077882,0.0001616944,0.000104599,0.0001423196,0.0003327187,0.0004552165],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003501422,"about_ca_system_score_gemma":0.0001830302,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006351552,"about_ca_topic_score_gemma":0.0007877778,"domain_scores_codex":[0.9998047,0.00006108081,0.00002511446,0.00003008414,0.00004688662,0.00003220372],"domain_scores_gemma":[0.9998394,0.00003088302,0.0000555754,0.00001759286,0.0000194984,0.00003692793],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006298349,0.00001076832,0.0001848035,0.00001956855,0.000003073828,0.00007302834,0.000007261121,0.0002069757,0.9983935,0.000107361,0.00002682392,0.0009038624],"study_design_scores_gemma":[0.00001166597,0.0001186774,0.001065886,0.00000279941,0.000008219737,0.000382409,0.00001264548,0.00128537,0.9951013,0.0000332926,0.001974211,0.000003667738],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9875128,0.0004304143,0.01002201,0.00007883347,0.00002399894,0.0000515794,0.0002518419,0.0001237277,0.001504817],"genre_scores_gemma":[0.9880165,0.0003250065,0.01046438,0.0000258009,0.000006458073,0.00001563,0.0003819298,0.00003572929,0.0007284752],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006351552,"threshold_uncertainty_score":0.002540529,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00503453312221061,"score_gpt":0.1863109782229051,"score_spread":0.1812764451006945,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}