{"id":"W1979089266","doi":"10.1021/bi0344230","title":"Engineering the Primary Substrate Specificity of <i>Streptomyces griseus</i> Trypsin","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; University of British Columbia","funders":"Genome British Columbia; University of Victoria","keywords":"Proteases; Streptomyces griseus; Bacillus subtilis; Trypsin; Mutant; Recombinant DNA; Serine; Protein engineering; Biochemistry; Chemistry; Enzyme; Substrate (aquarium); Streptomycetaceae; Actinomycetales; Stereochemistry; Biology; Streptomyces; Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000155807,0.0001537172,0.0001668197,0.00001010148,0.00004159605,0.00001650196,0.0002291947,0.0001344037,0.00008319901],"category_scores_gemma":[0.0001408746,0.0001159768,0.0001907465,0.0001184457,0.00008888965,0.000001787274,0.00004427313,0.00008661608,0.000004031688],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001323804,"about_ca_system_score_gemma":0.0000487869,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005446536,"about_ca_topic_score_gemma":7.328014e-7,"domain_scores_codex":[0.9991331,0.00001950693,0.0002360768,0.0002832837,0.0001380889,0.0001900095],"domain_scores_gemma":[0.999331,0.00002632649,0.00009828362,0.0004329765,0.00004698431,0.0000644561],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001040002,0.00004952278,0.001875012,0.00004159663,0.00005566368,8.562809e-7,0.000004027816,0.00002208269,0.9963642,0.00003200968,0.001342629,0.0002019996],"study_design_scores_gemma":[0.0001230044,0.000009061683,0.0006854604,0.00001328928,0.00002950029,0.000007376273,0.00003543119,0.00001055437,0.983902,0.00001258221,0.01502945,0.0001422674],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9911542,0.001315821,0.0001039209,0.00005658378,0.00003491861,0.00004243548,0.00002044004,0.000009592935,0.007262113],"genre_scores_gemma":[0.9985855,0.0001470976,0.0003610945,0.00005018012,0.0001401753,0.000007091994,0.00004868165,0.0000144725,0.0006457576],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01368682,"threshold_uncertainty_score":0.4729395,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00503453312221061,"score_gpt":0.1863109782229051,"score_spread":0.1812764451006945,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}