{"id":"W1979856832","doi":"10.3791/50612","title":"Permeabilization of Adhered Cells Using an Inert Gas Jet","year":2013,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Montreal Heart Institute; McGill University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Biophysics; Biomolecule; HeLa; Inert; Nanotechnology; Inert gas; Cell biology; Membrane; In vitro; Jet (fluid); Materials science; Cell culture; Chemistry; Biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005030814,0.0005798532,0.0004508372,0.0005550086,0.0005280474,0.0005885468,0.0006717152,0.0005926574,0.002328879],"category_scores_gemma":[0.000234312,0.0003273093,0.0006377128,0.0003247137,0.0004620315,0.0004370821,0.0004937589,0.0009408224,0.000853188],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003800046,"about_ca_system_score_gemma":0.0004151859,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008583741,"about_ca_topic_score_gemma":0.001234168,"domain_scores_codex":[0.9994206,0.00005790803,0.00007406535,0.0001499801,0.0002122732,0.0000850616],"domain_scores_gemma":[0.9998651,0.00004366078,0.00002680859,0.00003101764,0.00002205544,0.00001139872],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002219653,0.000008871344,0.00003219477,0.00005302611,0.000004084523,0.00006059782,0.00003872657,0.00004394657,0.9973357,0.0003429701,0.00007511975,0.00198253],"study_design_scores_gemma":[0.000007117038,0.00006028765,0.000355926,0.000009189898,0.00000992748,0.0001780622,0.0000155723,0.0004808759,0.9902676,0.00007318372,0.008532471,0.000009829898],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5866058,0.009777583,0.377324,0.0008933846,0.0006052604,0.001197466,0.001611853,0.002426584,0.01955814],"genre_scores_gemma":[0.7078823,0.01566575,0.2318685,0.0007169602,0.0001163167,0.001730076,0.00455933,0.0006903156,0.03677049],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002328879,"threshold_uncertainty_score":0.007790864,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03894124304512826,"score_gpt":0.4010552976561159,"score_spread":0.3621140546109876,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}