{"id":"W1981400366","doi":"10.1093/nar/gku797","title":"The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity","year":2014,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Biological and Environmental Research; Natural Sciences and Engineering Research Council of Canada; National Institutes of Health; Government of Canada; Genome Canada; Ontario Genomics; National Institute of General Medical Sciences; Ontario Genomics Institute; U.S. Department of Energy","keywords":"Nuclease; Biology; Sulfolobus solfataricus; Cleave; CRISPR; DNA; RecBCD; Helicase; Biochemistry; Micrococcal nuclease; Genetics; DNA repair; Gene; Archaea; Histone; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007339019,0.0002246627,0.0002147004,0.00004878619,0.0005351236,0.0001988105,0.0003879898,0.0003190059,0.00003574224],"category_scores_gemma":[0.0008930717,0.0001763034,0.00008397119,0.0001597991,0.0003051357,0.000008762681,0.0004892448,0.0005444451,0.000008159651],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005720312,"about_ca_system_score_gemma":0.00006288238,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008125602,"about_ca_topic_score_gemma":0.0013478,"domain_scores_codex":[0.9977027,0.0004368147,0.0001966156,0.0005381674,0.0004581427,0.0006675774],"domain_scores_gemma":[0.9988037,0.0001169935,0.00004981093,0.0005707961,0.0002044019,0.0002543505],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002335094,0.0000559665,0.001593305,0.00002308165,0.00009937114,0.000006370958,0.0001320832,0.00003389696,0.98166,0.00009000867,0.003039868,0.01303251],"study_design_scores_gemma":[0.004061539,0.001312949,0.1068352,0.00009277573,0.00005714797,0.00002636083,0.0006631092,0.01261443,0.7249097,0.001402717,0.147047,0.0009770232],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9954458,0.0004189376,0.00207285,0.0007638088,0.00008798077,0.0005915847,0.0001388366,0.00002526886,0.0004548865],"genre_scores_gemma":[0.998551,0.00008303521,0.0001722754,0.00006539945,0.0002836774,0.00005483627,0.0000497874,0.00005256595,0.0006874191],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2567504,"threshold_uncertainty_score":0.7189444,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01264214155763162,"score_gpt":0.3165874397956631,"score_spread":0.3039452982380315,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}