{"id":"W1981400366","doi":"10.1093/nar/gku797","title":"The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity","year":2014,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Biological and Environmental Research; Natural Sciences and Engineering Research Council of Canada; National Institutes of Health; Government of Canada; Genome Canada; Ontario Genomics; National Institute of General Medical Sciences; Ontario Genomics Institute; U.S. Department of Energy","keywords":"Nuclease; Biology; Sulfolobus solfataricus; Cleave; CRISPR; DNA; RecBCD; Helicase; Biochemistry; Micrococcal nuclease; Genetics; DNA repair; Gene; Archaea; Histone; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009634475,0.0003172836,0.0003519253,0.0001783417,0.0002224329,0.0003544076,0.0003253468,0.0004273886,0.0007237379],"category_scores_gemma":[0.000195867,0.0001649177,0.0002312186,0.0003443744,0.0001959043,0.0002078599,0.0001885738,0.0004565887,0.0003713488],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006518628,"about_ca_system_score_gemma":0.0004866382,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003019703,"about_ca_topic_score_gemma":0.001972693,"domain_scores_codex":[0.9998833,0.000009480575,0.000007297522,0.00002557018,0.00006208065,0.00001227971],"domain_scores_gemma":[0.9998914,0.000009330215,0.0000314696,0.000007885955,0.00001281025,0.000047186],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002060105,0.00008803395,0.003585488,0.0001504542,0.0000213268,0.0004064502,0.00006990862,0.001198271,0.9878575,0.0002464109,0.0005050153,0.005665267],"study_design_scores_gemma":[0.00008786978,0.000238251,0.03489423,0.00001567008,0.00003227195,0.002696879,0.00007924697,0.01046662,0.9347163,0.0001663382,0.01657064,0.00003560839],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9931775,0.001120759,0.003043592,0.0002726094,0.00002172183,0.00002005794,0.000920818,0.0002918971,0.001130991],"genre_scores_gemma":[0.9837282,0.0006862857,0.008435089,0.0001016603,0.00001055216,0.00001854964,0.004748885,0.00004171659,0.002229062],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003019703,"threshold_uncertainty_score":0.006004214,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01264214155763162,"score_gpt":0.3165874397956631,"score_spread":0.3039452982380315,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}