{"id":"W1981557743","doi":"10.1159/000068721","title":"O-Glycosylation of a Recombinant Carbohydrate-Binding Module Mutant Secreted by &lt;i&gt;Pichia pastoris&lt;/i&gt;","year":2003,"lang":"en","type":"article","venue":"Microbial Physiology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":36,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Pichia pastoris; Glycosylation; Mannose; Glycan; Biochemistry; Carbohydrate-binding module; Chemistry; Recombinant DNA; N-linked glycosylation; Mutant; Fusion protein; Yeast; Oligosaccharide; Glycoprotein; Molecular biology; Biology; Cellulose; Cellulase; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001137394,0.0004629993,0.000222989,0.0001057306,0.00008102541,0.0002291123,0.0002215925,0.0001905626,0.0004099178],"category_scores_gemma":[0.0001037983,0.0001065422,0.0002472477,0.0002530405,0.0001441961,0.0001180228,0.0001729054,0.0004469872,0.0002398926],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004164665,"about_ca_system_score_gemma":0.0002451915,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001761661,"about_ca_topic_score_gemma":0.001333815,"domain_scores_codex":[0.9998918,0.00001426618,0.00001128467,0.0000225335,0.00003151956,0.00002866869],"domain_scores_gemma":[0.9999328,0.000010719,0.00001844486,0.00001171732,0.000007163455,0.00001920815],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004721481,0.00001669844,0.0001487331,0.00001102267,0.000004679784,0.00004566599,0.000007027995,0.00004027355,0.9991336,0.00003834201,0.00002782506,0.0004790113],"study_design_scores_gemma":[0.0000105878,0.0001144134,0.004232805,0.000003137823,0.00001363557,0.0001710877,0.00001365407,0.001110875,0.9925328,0.00002055378,0.001772651,0.000003881433],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.996458,0.0002906786,0.001792325,0.00005659692,0.00002592464,0.00001310478,0.0004734203,0.00005678652,0.0008331407],"genre_scores_gemma":[0.9890921,0.0003069751,0.004154405,0.00006867187,0.000007809894,0.00002104248,0.002704389,0.00004739116,0.003597226],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001761661,"threshold_uncertainty_score":0.003502846,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007343969009796794,"score_gpt":0.2264479981404897,"score_spread":0.2191040291306929,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}