{"id":"W1982243149","doi":"10.3791/52687","title":"Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","field":"Immunology and Microbiology","cited_by":150,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Neutrophil extracellular traps; Immunofluorescence; Extracellular; Blot; DAPI; Cell culture; A549 cell; Staining; Biology; Chemistry; Cell biology; Molecular biology; In vitro; Immunology; Pathology; Biochemistry; Inflammation; Medicine; Gene; Antibody","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003690112,0.0007821296,0.0003644059,0.0003813395,0.0003266366,0.0003043616,0.0004509608,0.0004460933,0.004007651],"category_scores_gemma":[0.0002501417,0.000238941,0.0002793768,0.0002308135,0.0001547095,0.0002724651,0.0003736216,0.000719266,0.002672601],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001639308,"about_ca_system_score_gemma":0.000316714,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000494923,"about_ca_topic_score_gemma":0.00113077,"domain_scores_codex":[0.9996616,0.00006428186,0.00003766001,0.00006455481,0.0001285911,0.00004336473],"domain_scores_gemma":[0.999895,0.00001694561,0.00002198671,0.00002062372,0.00002560193,0.00001991928],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002145401,0.00006539049,0.0003045115,0.0002155292,0.00001248553,0.0001805481,0.0000287005,0.0003202604,0.9836454,0.0003643679,0.002173664,0.01247459],"study_design_scores_gemma":[0.00009551921,0.0007471839,0.005653592,0.00008407142,0.00006004212,0.002156193,0.00004862906,0.003981661,0.8944085,0.0005709311,0.09214206,0.00005165668],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4088556,0.02110482,0.5142577,0.001582353,0.001463533,0.006170067,0.02345191,0.003732971,0.01938109],"genre_scores_gemma":[0.4204913,0.01697795,0.480604,0.0008888041,0.0004218167,0.004964311,0.04151682,0.0003329121,0.03380212],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004007651,"threshold_uncertainty_score":0.01340687,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05968336933729345,"score_gpt":0.390556859319232,"score_spread":0.3308734899819385,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}