{"id":"W1982243149","doi":"10.3791/52687","title":"Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","field":"Immunology and Microbiology","cited_by":150,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Neutrophil extracellular traps; Immunofluorescence; Extracellular; Blot; DAPI; Cell culture; A549 cell; Staining; Biology; Chemistry; Cell biology; Molecular biology; In vitro; Immunology; Pathology; Biochemistry; Inflammation; Medicine; Gene; Antibody","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005085352,0.0002163174,0.0004522575,0.0002080889,0.0001848244,0.00004461729,0.0001739034,0.0001744233,0.0001639013],"category_scores_gemma":[0.00003937529,0.0001795757,0.0001083338,0.00009882865,0.00009809026,0.0002914664,0.00006161725,0.0002798452,0.00004432123],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006220953,"about_ca_system_score_gemma":0.00005698354,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001419133,"about_ca_topic_score_gemma":6.396435e-7,"domain_scores_codex":[0.9984962,0.0002873661,0.000629546,0.0002040507,0.0001060565,0.0002768253],"domain_scores_gemma":[0.9990337,0.00004080815,0.0004663813,0.0001516419,0.0002023338,0.0001050613],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004793349,0.0002089286,0.000182279,0.000005377837,0.0001686593,0.00003561947,0.001494621,0.000004675124,0.9943258,0.00163168,0.0007995387,0.0006634879],"study_design_scores_gemma":[0.006881709,0.00119321,0.0002493034,0.00006046892,0.00006513618,0.0002261186,0.001099456,0.00001001909,0.9717634,0.00100793,0.01723281,0.000210464],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.986297,0.008881307,0.00367109,0.0001139124,0.0006518459,0.0001532647,0.000003403505,0.00002178578,0.0002063994],"genre_scores_gemma":[0.9982834,0.00008778926,0.00074181,0.0001587177,0.0001087569,0.000004795571,0.00001816926,0.00002630081,0.0005702108],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02256243,"threshold_uncertainty_score":0.7322885,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05968336933729345,"score_gpt":0.390556859319232,"score_spread":0.3308734899819385,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}