{"id":"W1982496866","doi":"10.1016/j.pep.2005.05.012","title":"Production of pediocin PA-1 in the methylotrophic yeast Pichia pastoris reveals unexpected inhibition of its biological activity due to the presence of collagen-like material","year":2005,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Probiotics and Fermented Foods","field":"Agricultural and Biological Sciences","cited_by":45,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval; Biotechnology Research Institute","funders":"","keywords":"Pichia pastoris; Recombinant DNA; Pediococcus acidilactici; Bacteriocin; Yeast; Biochemistry; Pichia; Biological activity; Molecular cloning; Biology; Chemistry; Gene; Microbiology; Bacteria; Gene expression; In vitro; Antimicrobial; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001580947,0.0004099196,0.0003258453,0.0001513008,0.00009957504,0.0004419285,0.0002193956,0.0002687149,0.0004124433],"category_scores_gemma":[0.0001898032,0.0001715858,0.0002146117,0.0002238419,0.0002050724,0.0002507917,0.0002332283,0.0007791411,0.0002718704],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002719754,"about_ca_system_score_gemma":0.0001794913,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000714668,"about_ca_topic_score_gemma":0.0006658074,"domain_scores_codex":[0.9998686,0.00002043169,0.00001237296,0.00001947919,0.00005125413,0.00002775166],"domain_scores_gemma":[0.9998837,0.0000324085,0.000032272,0.00001414185,0.00001014184,0.00002732643],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003858637,0.000008076989,0.00005861401,0.00001232312,0.000002454598,0.00002932642,0.000003756188,0.00003802461,0.9993991,0.00002435762,0.00001048006,0.000374932],"study_design_scores_gemma":[0.000004749418,0.00004683481,0.0009930719,0.000002515785,0.00000688014,0.0001186283,0.000005960003,0.000492831,0.9976718,0.00001590891,0.000638405,0.000002356617],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9920347,0.001154295,0.005270624,0.0001767368,0.00002920063,0.00001760831,0.000453836,0.00008513196,0.0007778511],"genre_scores_gemma":[0.9924695,0.0005771658,0.003864438,0.00005443794,0.000009748481,0.00001791559,0.001226301,0.00003203737,0.001748487],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000714668,"threshold_uncertainty_score":0.001973331,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0267731521751221,"score_gpt":0.2401129754879372,"score_spread":0.2133398233128151,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}