{"id":"W1983031293","doi":"10.1016/j.bbamcr.2013.05.020","title":"Detection and quantification of endoplasmic reticulum stress in living cells using the fluorescent compound, Thioflavin T","year":2013,"lang":"en","type":"article","venue":"Biochimica et Biophysica Acta (BBA) - Molecular Cell Research","topic":"Endoplasmic Reticulum Stress and Disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":128,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University; Thrombosis and Atherosclerosis Research Institute","funders":"Canadian Institutes of Health Research","keywords":"Endoplasmic reticulum; Unfolded protein response; Cell biology; Thioflavin; Chemistry; STIM1; Biochemistry; Biology; Medicine; Pathology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005643632,0.0002695262,0.00024567,0.0001949166,0.0001879745,0.0001620012,0.0004455838,0.0001926724,0.00001170115],"category_scores_gemma":[0.0001337687,0.0002229074,0.0001351799,0.0003889352,0.0004932607,0.00002084934,0.0004233557,0.0004042571,0.000009446992],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003790893,"about_ca_system_score_gemma":0.0001413446,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007053349,"about_ca_topic_score_gemma":0.0000537728,"domain_scores_codex":[0.997022,0.0007763727,0.000427385,0.0006732636,0.0005531905,0.0005478349],"domain_scores_gemma":[0.9984419,0.0001527178,0.0001819326,0.000772204,0.0002841776,0.0001670502],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006202079,0.0003048164,0.0001596558,0.0001386461,0.00002984525,0.000005218223,0.0001616923,0.0001527209,0.9986802,0.00004121437,0.00005080529,0.0002131362],"study_design_scores_gemma":[0.0003763268,0.0001921927,0.002669521,0.0001137926,0.0000252925,0.000004262976,0.0005924747,0.005399437,0.9903048,0.00006032085,0.00002877084,0.000232842],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9977323,0.0004260786,0.0003924076,0.0002170505,0.00007471917,0.0008631884,0.00002994614,0.00001042888,0.000253841],"genre_scores_gemma":[0.9987453,0.0006451719,0.0002995343,0.00004511986,0.00005574451,0.00008076279,0.00004379274,0.00004690187,0.00003767853],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008375462,"threshold_uncertainty_score":0.9089898,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02125796149528223,"score_gpt":0.2925610288774932,"score_spread":0.271303067382211,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}