{"id":"W1983031293","doi":"10.1016/j.bbamcr.2013.05.020","title":"Detection and quantification of endoplasmic reticulum stress in living cells using the fluorescent compound, Thioflavin T","year":2013,"lang":"en","type":"article","venue":"Biochimica et Biophysica Acta (BBA) - Molecular Cell Research","topic":"Endoplasmic Reticulum Stress and Disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":128,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University; Thrombosis and Atherosclerosis Research Institute","funders":"Canadian Institutes of Health Research","keywords":"Endoplasmic reticulum; Unfolded protein response; Cell biology; Thioflavin; Chemistry; STIM1; Biochemistry; Biology; Medicine; Pathology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008104004,0.0009848276,0.0005434567,0.0006399482,0.0007152665,0.0006456154,0.0008942956,0.001304146,0.001008735],"category_scores_gemma":[0.000581758,0.0005049095,0.000410372,0.0005053214,0.001003946,0.001036611,0.0005214086,0.001399279,0.0007400637],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009149614,"about_ca_system_score_gemma":0.0007591303,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002432625,"about_ca_topic_score_gemma":0.002857287,"domain_scores_codex":[0.9996386,0.00007833272,0.00003014689,0.0001150353,0.00008953303,0.00004838206],"domain_scores_gemma":[0.9995067,0.0001479855,0.00006705667,0.00009113551,0.0001330393,0.00005400671],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005147939,0.00001240332,0.0000686631,0.0000370445,0.000004718329,0.00001872707,0.00003491528,0.00005120714,0.9988139,0.0002153735,0.00003590305,0.0006558187],"study_design_scores_gemma":[0.00001286186,0.00009043772,0.0005918086,0.000007474422,0.00001325148,0.0001128783,0.00003032626,0.001392497,0.9966431,0.0001917024,0.0009055902,0.000008026795],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6958592,0.002754137,0.2948577,0.000567587,0.0001623151,0.0001208042,0.000565556,0.00141677,0.00369596],"genre_scores_gemma":[0.7584334,0.004535959,0.2257303,0.0002822118,0.0000538156,0.000297627,0.001417853,0.0002759444,0.008972937],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002432625,"threshold_uncertainty_score":0.006638527,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02125796149528223,"score_gpt":0.2925610288774932,"score_spread":0.271303067382211,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}