{"id":"W1983424187","doi":"10.1016/j.jtice.2010.01.004","title":"Enhanced translocation of recombinant proteins via the Tat pathway with chaperones in Escherichia coli","year":2010,"lang":"en","type":"article","venue":"Journal of the Taiwan Institute of Chemical Engineers","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Canadian Institutes of Health Research; National Science Council","keywords":"Periplasmic space; Chromosomal translocation; Escherichia coli; Fusion protein; Green fluorescent protein; Twin-arginine translocation pathway; Inclusion bodies; Recombinant DNA; Proteolysis; Signal peptide; Chaperone (clinical); Biology; Cytoplasm; Chemistry; Cell biology; Biochemistry; Molecular biology; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003011901,0.0004359498,0.0003951564,0.0001681285,0.0002072998,0.0005637758,0.0002200912,0.0003601623,0.0004286244],"category_scores_gemma":[0.0003077909,0.0001623135,0.0003734619,0.0003371022,0.0002424528,0.0002935908,0.0002952421,0.0005872916,0.0003077871],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005385749,"about_ca_system_score_gemma":0.0003887671,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001744265,"about_ca_topic_score_gemma":0.001318945,"domain_scores_codex":[0.9997373,0.00007510671,0.00002985109,0.00003125237,0.0000646885,0.0000618095],"domain_scores_gemma":[0.9998389,0.00004097152,0.00003972364,0.00002369129,0.00003098242,0.00002567235],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001750466,0.00003776106,0.0001526032,0.00003233875,0.000006609445,0.0000541375,0.00002912517,0.0001613915,0.9984113,0.0001682071,0.00003027972,0.000741168],"study_design_scores_gemma":[0.00001544138,0.0001414459,0.000729951,0.000005715398,0.0000131778,0.0000830489,0.00003584009,0.00149824,0.9965262,0.00003804439,0.0009027228,0.00001021147],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9966782,0.000343236,0.002465325,0.00008725715,0.00002316744,0.00001181858,0.00009271881,0.00004098578,0.0002572027],"genre_scores_gemma":[0.995438,0.0002636422,0.002325193,0.00002626828,0.000004299292,0.00001073161,0.0001990732,0.00003500332,0.001697728],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001744265,"threshold_uncertainty_score":0.003907621,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003982137527536293,"score_gpt":0.1877276590091427,"score_spread":0.1837455214816064,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}