{"id":"W1984210811","doi":"10.1021/bp025572x","title":"Improving Glucose and Glutamine Metabolism of Human HEK 293 and <i>Trichoplusia</i> <i>ni</i> Insect Cells Engineered To Express a Cytosolic Pyruvate Carboxylase Enzyme","year":2003,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":68,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute","funders":"","keywords":"Pyruvate carboxylase; Biochemistry; Glutamine; Trichoplusia; Metabolic engineering; Biology; Metabolism; Enzyme; Metabolic pathway; Transfection; Flux (metallurgy); Glutamine synthetase; HEK 293 cells; Cytosol; Chemistry; Amino acid; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001676028,0.0003462774,0.0003951803,0.0002242004,0.0001640121,0.0000381967,0.000231696,0.0005346258,0.00000533499],"category_scores_gemma":[0.0001160542,0.0003285359,0.00007981091,0.0002951172,0.0003507805,0.00001118084,0.000370369,0.0001875364,0.000001480928],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001106248,"about_ca_system_score_gemma":0.00004782026,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007423862,"about_ca_topic_score_gemma":0.00001740044,"domain_scores_codex":[0.9982633,0.0000884451,0.000361491,0.0006920398,0.0001337537,0.0004609685],"domain_scores_gemma":[0.9989294,0.00001383699,0.0001775171,0.0005775659,0.00009067723,0.0002110042],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000730737,0.0001371238,0.0006523143,0.00009507478,0.00006568084,0.00001237621,0.00005849624,0.00001067024,0.9951876,0.0003922481,0.0003585313,0.002956812],"study_design_scores_gemma":[0.00123202,0.0006934296,0.0002957163,0.00003210163,0.00005913198,0.00003640591,0.0000527121,0.00002643444,0.987143,0.00007287337,0.009994531,0.0003615897],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9834028,0.01509197,0.0004610495,0.00009787195,0.0002118821,0.0005214062,0.0000711805,0.00008534216,0.00005644443],"genre_scores_gemma":[0.9972211,0.0006616843,0.00156378,0.000199913,0.00008167732,0.0001321003,0.00002003232,0.00004864835,0.00007107623],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01443029,"threshold_uncertainty_score":0.9999167,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006142749850909381,"score_gpt":0.2232690243810486,"score_spread":0.2171262745301392,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}