{"id":"W1984210811","doi":"10.1021/bp025572x","title":"Improving Glucose and Glutamine Metabolism of Human HEK 293 and <i>Trichoplusia</i> <i>ni</i> Insect Cells Engineered To Express a Cytosolic Pyruvate Carboxylase Enzyme","year":2003,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":68,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute","funders":"","keywords":"Pyruvate carboxylase; Biochemistry; Glutamine; Trichoplusia; Metabolic engineering; Biology; Metabolism; Enzyme; Metabolic pathway; Transfection; Flux (metallurgy); Glutamine synthetase; HEK 293 cells; Cytosol; Chemistry; Amino acid; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002079634,0.000254549,0.0002710096,0.0001318813,0.0001463513,0.0004140204,0.0001815193,0.0001885607,0.0005660903],"category_scores_gemma":[0.0001276834,0.00009913496,0.0002979585,0.0002689504,0.00009631066,0.0001871155,0.0001637842,0.0002918655,0.0003518373],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002356952,"about_ca_system_score_gemma":0.0001900879,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001084522,"about_ca_topic_score_gemma":0.001096476,"domain_scores_codex":[0.9998494,0.0000263854,0.00002185537,0.00003588999,0.00005002496,0.00001640812],"domain_scores_gemma":[0.9999545,0.000008177808,0.00001134082,0.000006749965,0.0000122548,0.000007052785],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006735991,0.00001061942,0.0001700875,0.00002481564,0.000004306631,0.00002571358,0.00001105086,0.00004239809,0.9982561,0.00005136817,0.00002580918,0.001310306],"study_design_scores_gemma":[0.000007719194,0.0001242912,0.002219644,0.000003088103,0.00002347094,0.0002302091,0.00002473501,0.000753146,0.9936265,0.00002213146,0.002961074,0.000004026504],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.97859,0.002868896,0.01403606,0.0001424737,0.00005854728,0.00006543541,0.001135978,0.0001200546,0.002982516],"genre_scores_gemma":[0.9620122,0.003400193,0.02640002,0.00007266012,0.00001160819,0.00005192106,0.003770604,0.00007213312,0.004208577],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001084522,"threshold_uncertainty_score":0.002156377,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006142749850909381,"score_gpt":0.2232690243810486,"score_spread":0.2171262745301392,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}