{"id":"W1984215270","doi":"10.1016/j.pep.2005.10.028","title":"Expression and purification of sea raven type II antifreeze protein from Drosophila melanogaster S2 cells","year":2005,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Physiological and biochemical adaptations","field":"Environmental Science","cited_by":20,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; Ontario Institute for Cancer Research; Queen's University","funders":"Canada Research Chairs; Government of Canada","keywords":"Drosophila melanogaster; Antifreeze protein; Biology; Drosophila (subgenus); Protein expression; Cell biology; Schneider 2 cells; Genetics; Gene; RNA interference","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002863592,0.0007256251,0.0004014026,0.0003606724,0.000336419,0.0004107138,0.0005643541,0.0002947781,0.001361932],"category_scores_gemma":[0.0001817812,0.000385707,0.0004920114,0.0004891064,0.0002557917,0.0003124698,0.0003172939,0.0009685917,0.001387523],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004784295,"about_ca_system_score_gemma":0.0002802751,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002675798,"about_ca_topic_score_gemma":0.004559054,"domain_scores_codex":[0.9998423,0.00001929378,0.00001999194,0.00004843809,0.00003776725,0.00003217326],"domain_scores_gemma":[0.9998241,0.00003280628,0.00002476154,0.0000439604,0.0000405449,0.00003383378],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004557134,0.00001305389,0.000137581,0.00003121007,0.000005782887,0.00002758677,0.00002587282,0.00002604215,0.9989958,0.0000789817,0.0001198664,0.0004925546],"study_design_scores_gemma":[0.00002246125,0.0000861147,0.00554633,0.000007366381,0.00001977416,0.0001931215,0.00004052642,0.0007043984,0.9842988,0.00004641997,0.009026323,0.000008267999],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9495285,0.002564514,0.03421972,0.0004852909,0.0002414808,0.0002730098,0.007431311,0.0006032479,0.004652876],"genre_scores_gemma":[0.8544995,0.003261072,0.05706827,0.0004150992,0.0001210997,0.0003366581,0.04347465,0.0006630822,0.04016061],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002675798,"threshold_uncertainty_score":0.00532037,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0174369775205987,"score_gpt":0.221989246762376,"score_spread":0.2045522692417773,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}