{"id":"W1985625642","doi":"10.1186/1472-6750-11-114","title":"Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII","year":2011,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Hemophilia Treatment and Research","field":"Medicine","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Biotechnology Research Institute","funders":"National Research Council Canada; Financiadora de Estudos e Projetos; Universidade de São Paulo; Fundação de Amparo à Pesquisa do Estado de São Paulo","keywords":"Transfection; HEK 293 cells; Biology; Cell culture; Recombinant DNA; Economic shortage; Immunology; Molecular biology; Cell biology; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001050574,0.0005050901,0.001171546,0.0003564284,0.0003864793,0.0009814902,0.0008827717,0.000759764,0.003142128],"category_scores_gemma":[0.0006082229,0.0003042592,0.0006683656,0.0008717012,0.0004943468,0.0005078773,0.0004222764,0.001632926,0.002040466],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006375026,"about_ca_system_score_gemma":0.0004341284,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006803027,"about_ca_topic_score_gemma":0.0007842791,"domain_scores_codex":[0.9988142,0.0001960746,0.0002015579,0.0002515047,0.0004227259,0.0001139947],"domain_scores_gemma":[0.999592,0.0001342925,0.00006768623,0.00008190666,0.00009319033,0.0000309394],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001001504,0.00007326126,0.0000833963,0.0001117799,0.00001085601,0.00005755576,0.00006338867,0.0001319642,0.9971071,0.0003616576,0.0002483244,0.001650616],"study_design_scores_gemma":[0.00002861975,0.0001904405,0.0006720769,0.00001269153,0.00003521243,0.0001613278,0.00001877201,0.003012611,0.9871165,0.00008796272,0.008650506,0.00001333426],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7266315,0.004619438,0.2409959,0.0008272753,0.001012295,0.001591986,0.008449998,0.001685478,0.0141862],"genre_scores_gemma":[0.7752998,0.003202571,0.177679,0.000370983,0.0001384394,0.001455325,0.01952323,0.0004663863,0.02186412],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003142128,"threshold_uncertainty_score":0.01051146,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04693670554921537,"score_gpt":0.2913800458030533,"score_spread":0.244443340253838,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}