{"id":"W1985625642","doi":"10.1186/1472-6750-11-114","title":"Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII","year":2011,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Hemophilia Treatment and Research","field":"Medicine","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Biotechnology Research Institute","funders":"National Research Council Canada; Financiadora de Estudos e Projetos; Universidade de São Paulo; Fundação de Amparo à Pesquisa do Estado de São Paulo","keywords":"Transfection; HEK 293 cells; Biology; Cell culture; Recombinant DNA; Economic shortage; Immunology; Molecular biology; Cell biology; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001955577,0.0001249599,0.0003056135,0.0002845822,0.00006629401,9.674562e-7,0.0001105595,0.0004417345,0.0000462339],"category_scores_gemma":[0.00005234376,0.00009101213,0.0001474126,0.0002955817,0.0002568164,0.00001702459,0.00001964313,0.000182658,0.000003489288],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004298304,"about_ca_system_score_gemma":0.00004770599,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000378579,"about_ca_topic_score_gemma":0.00007514258,"domain_scores_codex":[0.9990007,0.00002870027,0.0002823208,0.0002961099,0.0001687335,0.0002233982],"domain_scores_gemma":[0.9992733,0.00001219282,0.00007141926,0.0004406247,0.0001593182,0.00004312288],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004840053,0.001107124,0.001355043,0.0007284662,0.00003752106,0.000003041677,0.000960279,0.000006363124,0.9915242,0.0009829726,0.0002878023,0.002523251],"study_design_scores_gemma":[0.002466291,0.003342105,0.006568301,0.00007839258,0.0001206463,0.0000246004,0.0004584416,0.00007114636,0.9861914,0.0002438227,0.0003596792,0.00007515451],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.994725,0.0002441394,0.001952882,0.0008619477,0.0001576938,0.001379007,0.0000149542,0.00008973188,0.0005745688],"genre_scores_gemma":[0.9946364,0.00008525501,0.004478023,0.00000465247,0.00003686215,0.00003296492,0.00001773226,0.00001665119,0.0006914539],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005332711,"threshold_uncertainty_score":0.3711367,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04693670554921537,"score_gpt":0.2913800458030533,"score_spread":0.244443340253838,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}