{"id":"W1985701837","doi":"10.1007/s002990100321","title":"Multiplex PCR combining transgene and S-allele control primers to simultaneously confirm cultivar identity and transformation in apple","year":2001,"lang":"en","type":"article","venue":"Plant Cell Reports","topic":"Plant Virus Research Studies","field":"Agricultural and Biological Sciences","cited_by":7,"is_retracted":false,"has_abstract":false,"ca_institutions":"Agriculture and Agri-Food Canada","funders":"","keywords":"Biology; Transformation (genetics); Genetics; Allele; Cultivar; Transgene; Multiplex polymerase chain reaction; Multiplex; Variants of PCR; Polymerase chain reaction; Cloning (programming); Gene; Botany; Molecular biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00103675,0.001102579,0.001081957,0.001543589,0.0006759227,0.0007231599,0.0008053818,0.0008348957,0.003597039],"category_scores_gemma":[0.0008221607,0.001336454,0.001368998,0.0006350635,0.0005869559,0.0007865274,0.0005854989,0.001778173,0.001680797],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007806676,"about_ca_system_score_gemma":0.0007813062,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001229516,"about_ca_topic_score_gemma":0.004610586,"domain_scores_codex":[0.998768,0.0001148981,0.0001493571,0.0006514624,0.0001504232,0.0001658727],"domain_scores_gemma":[0.998774,0.0004353714,0.0002325033,0.0001598552,0.0001757499,0.0002225215],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007525011,0.00004367457,0.000317095,0.00004568038,0.00001081412,0.00006650433,0.0000759388,0.00004122332,0.9968394,0.000179952,0.0001249813,0.002179567],"study_design_scores_gemma":[0.00008130898,0.0005358564,0.0103548,0.00004167111,0.0001904292,0.0008414967,0.0001528726,0.002155775,0.9801548,0.0002410018,0.005218745,0.00003119022],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6927224,0.001324505,0.2926939,0.0005621155,0.0004456973,0.001071615,0.00287646,0.002450541,0.005852843],"genre_scores_gemma":[0.6577681,0.001248937,0.3079442,0.0008668072,0.0001615016,0.001638374,0.009053195,0.001028524,0.02029033],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003597039,"threshold_uncertainty_score":0.01203334,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0297013918746526,"score_gpt":0.2515851143277038,"score_spread":0.2218837224530512,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}