{"id":"W1986299293","doi":"10.1186/1472-6750-9-75","title":"Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR","year":2009,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":104,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"Genome Canada","keywords":"Biology; Primer (cosmetics); Oligonucleotide; Genetics; Primer dimer; Computational biology; Polymerase chain reaction; In silico PCR; Primer binding site; Molecular biology; DNA; Multiple displacement amplification; Gene; Multiplex polymerase chain reaction; DNA extraction; Reverse transcriptase","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002737723,0.0001049788,0.000134432,0.00005598589,0.00003584649,0.000002124801,0.0001822156,0.000308025,0.000005426122],"category_scores_gemma":[0.0001421082,0.00007239595,0.00009987075,0.0001250683,0.0003006093,0.000001302545,0.00006031821,0.00007255273,7.572879e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001548648,"about_ca_system_score_gemma":0.00007243509,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003890633,"about_ca_topic_score_gemma":0.00001297251,"domain_scores_codex":[0.9993,0.00009508243,0.0001735212,0.0002179846,0.0001009899,0.0001124161],"domain_scores_gemma":[0.9992515,0.00001137218,0.0001703629,0.000438432,0.0001114679,0.00001679796],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000309882,0.0001155812,0.0009006089,0.000001945696,0.00003049333,4.111194e-8,0.00001052906,0.00002621857,0.9877377,0.004957477,0.0001210028,0.006067483],"study_design_scores_gemma":[0.0002472417,0.001199349,0.0264739,0.0000120257,0.00003951897,0.000006626342,0.00002152386,0.0001485202,0.968449,0.003137606,0.0001901259,0.00007456957],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9940964,0.0007421212,0.003807916,0.0005562587,0.00001512041,0.0003218547,0.00001775647,0.00001492806,0.0004276517],"genre_scores_gemma":[0.9944574,0.00005663862,0.005373511,0.00005513862,0.00001000282,0.0000124766,0.0000120856,0.000007006241,0.00001579708],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02557329,"threshold_uncertainty_score":0.2952221,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03539943908644091,"score_gpt":0.3212186407361528,"score_spread":0.2858192016497119,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}