{"id":"W1986347124","doi":"10.1038/sj.gt.3301532","title":"Transfection of large plasmids in primary human myoblasts","year":2001,"lang":"en","type":"article","venue":"Gene Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":87,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval","funders":"Medical Research Council Canada; Muscular Dystrophy Association","keywords":"Transfection; Molecular biology; Biology; Plasmid; Duchenne muscular dystrophy; Genetic enhancement; Gene delivery; Myocyte; Polyethylenimine; Dystrophin; Cell culture; Cell biology; DNA; Gene; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003994799,0.0001378894,0.000165053,0.0001105679,0.0000518297,0.000006905098,0.0001912933,0.0001332367,0.0001343375],"category_scores_gemma":[0.000003707227,0.0001364423,0.00009117786,0.0001978447,0.00005553422,0.000003734774,0.00002310191,0.0001008864,0.000007905725],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002683544,"about_ca_system_score_gemma":0.00006818719,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002281269,"about_ca_topic_score_gemma":0.0003904355,"domain_scores_codex":[0.9988708,0.0001253446,0.0002186058,0.0002768025,0.0001778862,0.0003305893],"domain_scores_gemma":[0.9995203,0.000006468285,0.00003888203,0.0003345989,0.00004870216,0.00005107422],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002688756,0.0002600138,0.005753798,0.000007480716,0.00004199876,0.00000411911,0.00007806746,0.00008418106,0.9803552,0.000008469765,0.0001202025,0.01059773],"study_design_scores_gemma":[0.002728707,0.003180469,0.01739278,0.000006715519,0.000001828259,0.000007909322,0.00001413626,0.00001165841,0.9711046,0.00008571361,0.005322595,0.0001429597],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9969664,0.001347973,0.0002409224,0.0000688054,0.00005032547,0.0002516255,0.00001396235,0.00001235357,0.001047635],"genre_scores_gemma":[0.9961412,0.002854989,0.00005732236,0.0002663731,0.0001389543,0.00003228807,0.0001070742,0.00003216842,0.0003696364],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01163898,"threshold_uncertainty_score":0.5563954,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01675311841658245,"score_gpt":0.3043335316002507,"score_spread":0.2875804131836682,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}