{"id":"W1986347124","doi":"10.1038/sj.gt.3301532","title":"Transfection of large plasmids in primary human myoblasts","year":2001,"lang":"en","type":"article","venue":"Gene Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":87,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval","funders":"Medical Research Council Canada; Muscular Dystrophy Association","keywords":"Transfection; Molecular biology; Biology; Plasmid; Duchenne muscular dystrophy; Genetic enhancement; Gene delivery; Myocyte; Polyethylenimine; Dystrophin; Cell culture; Cell biology; DNA; Gene; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001382139,0.0007181602,0.001081714,0.0007104851,0.0006899981,0.0008233692,0.0005896051,0.0008292276,0.003703651],"category_scores_gemma":[0.0006020026,0.0005190562,0.0008590074,0.0008674596,0.0004806962,0.0005145298,0.0007364032,0.001839229,0.002112019],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007047286,"about_ca_system_score_gemma":0.0007400707,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002069269,"about_ca_topic_score_gemma":0.003267911,"domain_scores_codex":[0.9993101,0.000136225,0.00008114157,0.000137499,0.0001685738,0.0001664157],"domain_scores_gemma":[0.9995261,0.0002321686,0.00002703572,0.00009649509,0.00004935923,0.00006888135],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000181794,0.00008942236,0.0001400716,0.0001498784,0.00002233569,0.00008658388,0.0001448523,0.0001443341,0.993322,0.001062852,0.0004522222,0.004203649],"study_design_scores_gemma":[0.00007732805,0.0003740992,0.001733743,0.00002578077,0.00006859672,0.0002131298,0.00004400489,0.001079988,0.983744,0.0002238263,0.0124065,0.000009080568],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7446555,0.01007588,0.2084027,0.001036058,0.0013151,0.002436633,0.01099136,0.002197918,0.0188887],"genre_scores_gemma":[0.7891386,0.005700604,0.1249925,0.0004799712,0.0002194725,0.001694787,0.02477157,0.0005611319,0.05244133],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003703651,"threshold_uncertainty_score":0.01238996,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01675311841658245,"score_gpt":0.3043335316002507,"score_spread":0.2875804131836682,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}