{"id":"W1986376155","doi":"10.1016/j.jbiotec.2005.03.004","title":"A multiwavelength fluorescence probe: Is one probe capable for on-line monitoring of recombinant protein production and biomass activity?","year":2005,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":46,"is_retracted":false,"has_abstract":false,"ca_institutions":"Polytechnique Montréal","funders":"Canada Research Chairs","keywords":"Pichia pastoris; Green fluorescent protein; Fluorescence; Bioreactor; Yeast; Tryptophan; Recombinant DNA; NAD+ kinase; Chemistry; Biomass (ecology); Fluorescence spectroscopy; Biochemistry; In situ; Biology; Chromatography; Biophysics; Enzyme; Botany; Optics; Amino acid","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001293095,0.0009435056,0.001224891,0.0005191871,0.0004320356,0.001326406,0.001613927,0.003591078,0.002081604],"category_scores_gemma":[0.002189925,0.0005385718,0.0005011869,0.0004854058,0.001186098,0.00350289,0.0006702352,0.002144591,0.001378871],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007468655,"about_ca_system_score_gemma":0.0005042607,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002901072,"about_ca_topic_score_gemma":0.0003879775,"domain_scores_codex":[0.9991615,0.0001547481,0.00003276434,0.0002741418,0.0002542329,0.000122693],"domain_scores_gemma":[0.9990114,0.0003578661,0.0001715697,0.0001223263,0.0001330469,0.0002038041],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000107556,0.00004662713,0.0004334072,0.00007008599,0.000008443248,0.00005864868,0.00003073899,0.0000522362,0.9856696,0.00108296,0.0002319306,0.01220777],"study_design_scores_gemma":[0.0000317782,0.0004527285,0.002537385,0.00003612009,0.00004269093,0.002446133,0.0001081937,0.004562893,0.9793922,0.001751528,0.008580062,0.00005825889],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4415314,0.009287775,0.5291488,0.01008931,0.000496754,0.0002011625,0.0003933334,0.001614342,0.007237014],"genre_scores_gemma":[0.7365739,0.004890569,0.2468991,0.002962675,0.0002029225,0.0003024001,0.000455591,0.0002891003,0.007423763],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003591078,"threshold_uncertainty_score":0.00696367,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02133749525783132,"score_gpt":0.2790118972033261,"score_spread":0.2576744019454947,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}