{"id":"W1986537945","doi":"10.1107/s1744309110034378","title":"Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of intracellular growth locus E (IglE) protein from<i>Francisella tularensis</i>subsp.<i>novicida</i>","year":2010,"lang":"en","type":"article","venue":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","topic":"Bacillus and Francisella bacterial research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Victoria","funders":"Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Francisella tularensis; Francisella; Virulence; Biology; Periplasmic space; Secretion; Tularemia; Microbiology; Locus (genetics); Leptospira; Pathogenicity island; Intracellular parasite; Virulence factor; Bacteria; Genetics; Virology; Gene; Escherichia coli; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004052702,0.0004589155,0.0005244226,0.0003211563,0.0005269668,0.0004594535,0.0006380377,0.0005378405,0.0005749454],"category_scores_gemma":[0.0006789251,0.0002368793,0.0003385701,0.001044668,0.0002775942,0.0003116769,0.0002331888,0.0010699,0.0006358969],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007748588,"about_ca_system_score_gemma":0.0004667797,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.005899765,"about_ca_topic_score_gemma":0.005825947,"domain_scores_codex":[0.9997521,0.00004009836,0.00002929379,0.00003809497,0.00008207007,0.00005822049],"domain_scores_gemma":[0.9997851,0.00004951419,0.00002937857,0.00002441876,0.00007823505,0.00003344257],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004765265,0.0002334919,0.001757158,0.000362175,0.00003315562,0.0006993876,0.000376916,0.002328802,0.9811406,0.000688876,0.001634571,0.0102685],"study_design_scores_gemma":[0.000067078,0.0003489872,0.02388707,0.0000560663,0.00006430608,0.001491519,0.0005084539,0.006511174,0.9366641,0.0003759283,0.02996754,0.00005774418],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9740771,0.004912914,0.01105714,0.0009043214,0.00009385624,0.0001402855,0.005749181,0.0001963917,0.002868767],"genre_scores_gemma":[0.8936839,0.01033576,0.04290042,0.0002812016,0.00004574465,0.0002202223,0.04371127,0.0002525641,0.008569085],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005899765,"threshold_uncertainty_score":0.01173085,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007800398873227685,"score_gpt":0.2470927732189046,"score_spread":0.2392923743456769,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}