{"id":"W1986593253","doi":"10.1021/bi026481b","title":"Backbone Dynamics of Green Fluorescent Protein and the Effect of Histidine 148 Substitution","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":57,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Green fluorescent protein; Chromophore; Chemistry; Fluorescence; Protein dynamics; Molecular dynamics; Microsecond; Photochemistry; Proton NMR; Protein folding; Biophysics; Stereochemistry; Computational chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002859769,0.0001526378,0.0002078289,0.00001502366,0.00003639736,0.00000380655,0.0001461236,0.0001535173,0.000005113498],"category_scores_gemma":[0.0002644848,0.0001109668,0.00007158732,0.00007260967,0.0006428604,0.000002332857,0.00006815195,0.00008197517,2.960828e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000026864,"about_ca_system_score_gemma":0.00004080225,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002850712,"about_ca_topic_score_gemma":0.000004478168,"domain_scores_codex":[0.9992241,0.00006543897,0.00022885,0.0002361013,0.0001039494,0.0001415246],"domain_scores_gemma":[0.9993432,0.00001595168,0.0001582815,0.0003784003,0.00006646044,0.00003774715],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003200959,0.00002050846,0.001840454,0.000219248,0.00002039262,6.190666e-7,0.000005573182,0.000001431173,0.996439,0.0005932435,0.0000475118,0.0004918752],"study_design_scores_gemma":[0.0009579046,0.0001860466,0.00007667511,0.00006595722,0.00002301171,0.00001218025,0.000009672482,0.00004741834,0.9982184,0.00009406788,0.0002009383,0.0001077792],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9923847,0.001755246,0.005108335,0.00004508373,0.00002156162,0.0003908281,0.00003200574,0.00001112115,0.000251169],"genre_scores_gemma":[0.99499,0.00009643961,0.004518987,0.000007092895,0.00002480162,0.00003627659,0.0000700271,0.00001368495,0.0002427143],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002605326,"threshold_uncertainty_score":0.4525096,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002521439014364999,"score_gpt":0.2240914393204105,"score_spread":0.2215700003060455,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}