{"id":"W1986682991","doi":"10.1016/j.pep.2014.12.013","title":"Expression and purification of human WWP2 HECT domain in Escherichia coli","year":2014,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"Ministry of Science and Technology of the People's Republic of China; National Natural Science Foundation of China","keywords":"Escherichia coli; Circular dichroism; Ubiquitin ligase; Affinity chromatography; Ubiquitin; Biology; Biochemistry; Chemistry; Chromatography; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005146739,0.0007391043,0.0007102059,0.0004662019,0.0004521577,0.0006908245,0.0006427563,0.000462048,0.0008232406],"category_scores_gemma":[0.0003693749,0.0004410321,0.0004312844,0.001464335,0.0002182747,0.0003472811,0.0004830633,0.0009587954,0.001361237],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006783974,"about_ca_system_score_gemma":0.0004806911,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002785496,"about_ca_topic_score_gemma":0.002444932,"domain_scores_codex":[0.9995474,0.00008575286,0.00006238974,0.00007242287,0.0001298835,0.0001020602],"domain_scores_gemma":[0.9998449,0.00003278125,0.0000175841,0.00003133694,0.00004448762,0.00002880228],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003456351,0.0001553222,0.0008831932,0.0002384446,0.0000445462,0.0002797888,0.00009823748,0.0005491462,0.9913148,0.0006434569,0.0007993807,0.004648031],"study_design_scores_gemma":[0.00004645731,0.0001226024,0.005796715,0.00002542935,0.00003973908,0.0003979671,0.00009275954,0.002611726,0.9765422,0.0001983424,0.01410272,0.00002343093],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9011112,0.004747324,0.06540044,0.00118377,0.0003563311,0.0006196414,0.01976285,0.0008202464,0.00599831],"genre_scores_gemma":[0.8287005,0.005152348,0.08209606,0.0005775358,0.00008941352,0.0005626967,0.05750753,0.0002987625,0.02501508],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002785496,"threshold_uncertainty_score":0.005538583,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01366102716397313,"score_gpt":0.2518647686082757,"score_spread":0.2382037414443026,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}