{"id":"W1986682991","doi":"10.1016/j.pep.2014.12.013","title":"Expression and purification of human WWP2 HECT domain in Escherichia coli","year":2014,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"Ministry of Science and Technology of the People's Republic of China; National Natural Science Foundation of China","keywords":"Escherichia coli; Circular dichroism; Ubiquitin ligase; Affinity chromatography; Ubiquitin; Biology; Biochemistry; Chemistry; Chromatography; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005670032,0.0001513926,0.0001637723,0.0000767249,0.0001193122,0.00002713778,0.0001198062,0.0002069729,0.00001888543],"category_scores_gemma":[0.00009727447,0.0001369396,0.00002894358,0.00008650687,0.00009295757,0.00001469455,0.00007132506,0.0001113644,0.000002184702],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006905635,"about_ca_system_score_gemma":0.00001843076,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001899204,"about_ca_topic_score_gemma":0.000005452417,"domain_scores_codex":[0.9987096,0.0002766407,0.0003138873,0.000403215,0.0001368591,0.0001597995],"domain_scores_gemma":[0.9992897,0.00001390216,0.0002113156,0.0003444304,0.00005623203,0.00008444822],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008643288,0.00007425204,0.001122276,0.00009667276,0.000002310716,1.274208e-7,0.0002460439,0.000001440408,0.9934013,0.0009557731,0.00007510391,0.003938272],"study_design_scores_gemma":[0.0007103438,0.0001943335,0.0071281,0.0001597696,0.000003133287,0.000001277582,0.0001015602,0.00006423736,0.9834977,0.001662195,0.006314393,0.0001629654],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9943866,0.0008264574,0.00279907,0.0001441024,0.00002431212,0.0006951414,0.000007088992,0.00001564718,0.001101654],"genre_scores_gemma":[0.9938769,0.0001234963,0.005296197,0.0000397501,0.00007955841,0.0002580659,0.0001015254,0.00001802731,0.000206534],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.009903603,"threshold_uncertainty_score":0.5584233,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01366102716397313,"score_gpt":0.2518647686082757,"score_spread":0.2382037414443026,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}