{"id":"W1986724556","doi":"10.1016/j.plaphy.2008.12.019","title":"Directed evolution of acyl-CoA:diacylglycerol acyltransferase: Development and characterization of Brassica napus DGAT1 mutagenized libraries","year":2009,"lang":"en","type":"article","venue":"Plant Physiology and Biochemistry","topic":"Lipid metabolism and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":59,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Diacylglycerol kinase; Brassica; Mutant; Saccharomyces cerevisiae; Biochemistry; Acyl-CoA; Yeast; Directed evolution; Biology; Enzyme; Arabidopsis; Acyltransferase; Arabidopsis thaliana; Metabolic engineering; Flux (metallurgy); Gene; Chemistry; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004930769,0.001161716,0.0006188122,0.001330023,0.000363436,0.0006145768,0.0007860265,0.0005289781,0.001819258],"category_scores_gemma":[0.0004408329,0.0006454631,0.00068467,0.0009924802,0.0004084632,0.0002461742,0.0007044859,0.001411692,0.001354993],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009913131,"about_ca_system_score_gemma":0.0005450602,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00246345,"about_ca_topic_score_gemma":0.005082677,"domain_scores_codex":[0.9995863,0.00007156839,0.00004787722,0.00008849375,0.0001331505,0.0000726271],"domain_scores_gemma":[0.9995676,0.0001281195,0.00009718862,0.00006121005,0.00005922105,0.00008663777],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005875867,0.0000198148,0.00006340053,0.00002599529,0.000006996118,0.00002887679,0.00001937925,0.0001100054,0.9981871,0.0001143543,0.00003000072,0.001335205],"study_design_scores_gemma":[0.00002107405,0.00006732038,0.0009380569,0.000006119577,0.00003780677,0.000155615,0.0000188933,0.000708073,0.9954046,0.00003902985,0.002593228,0.00001003788],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9112729,0.001089102,0.07538067,0.0005158061,0.000113974,0.0006283355,0.005589574,0.001579922,0.003829722],"genre_scores_gemma":[0.9196708,0.001655157,0.04552963,0.0002587911,0.00003034575,0.000318181,0.01618076,0.001181284,0.01517502],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00246345,"threshold_uncertainty_score":0.007192552,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004143957081400686,"score_gpt":0.1806581227043704,"score_spread":0.1765141656229697,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}