{"id":"W1987274897","doi":"10.1016/s0006-3495(00)76688-4","title":"Protonation of Lysine Residues Inverts Cation/Anion Selectivity in a Model Channel","year":2000,"lang":"en","type":"article","venue":"Biophysical Journal","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":45,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"","keywords":"Protonation; Chemistry; Selectivity; Membrane; Deprotonation; Ion; Ion channel; Alamethicin; Electrostatics; Biophysics; Stereochemistry; Crystallography; Lipid bilayer; Organic chemistry; Biochemistry; Physical chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008400036,0.00008322331,0.000112008,0.00004221603,0.0000381771,0.00001060225,0.00008295961,0.00007959446,0.00001777802],"category_scores_gemma":[0.00001486478,0.00006907278,0.00006206048,0.000111453,0.00003374186,0.00001012425,0.00001330221,0.0001058071,0.000001899164],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000160356,"about_ca_system_score_gemma":0.00007640226,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001915176,"about_ca_topic_score_gemma":0.00002032367,"domain_scores_codex":[0.9993929,0.00004263302,0.0001695425,0.0001381293,0.000135827,0.0001210058],"domain_scores_gemma":[0.999706,0.000002674857,0.00006602995,0.0000988438,0.00007975691,0.00004673504],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001188545,0.0001401282,0.0003787272,0.000008434693,0.000007200538,0.00000118794,0.00005354777,0.0009267718,0.99437,0.00001804497,0.00007814815,0.003898943],"study_design_scores_gemma":[0.0004431633,0.0001555887,0.01085593,0.00001450421,0.00001123437,0.00002488934,0.000005512593,0.003150661,0.9849631,0.0001614607,0.0001246825,0.00008923015],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9991628,0.000007426777,0.0003219852,0.0001211725,0.00003141045,0.0001460226,0.000005177877,0.000003249151,0.0002007658],"genre_scores_gemma":[0.9991594,0.00004741884,0.0001636301,0.00005235511,0.0003276032,0.0000118348,0.00002057804,0.000008038025,0.0002091598],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0104772,"threshold_uncertainty_score":0.2816706,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01071235303487799,"score_gpt":0.2552797584523898,"score_spread":0.2445674054175118,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}