{"id":"W1987514868","doi":"10.1016/j.pep.2009.06.016","title":"Site-directed mutagenesis, purification and assay of Saccharomyces cerevisiae RNA polymerase II","year":2009,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":21,"is_retracted":false,"has_abstract":false,"ca_institutions":"Montreal Clinical Research Institute","funders":"National Institute of General Medical Sciences; Canadian Institutes of Health Research","keywords":"Saccharomyces cerevisiae; Biology; Mutant; Protein subunit; Polymerase; Biochemistry; Tandem affinity purification; RNA polymerase; Mutagenesis; RNA; Site-directed mutagenesis; Yeast; Genetics; DNA; Enzyme; Gene; Affinity chromatography","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009174088,0.001048537,0.0008405397,0.0006685054,0.0005690587,0.0006416081,0.0009578532,0.0005295264,0.001253381],"category_scores_gemma":[0.0009025842,0.0009288568,0.0006732736,0.0007384788,0.0006047232,0.0002998186,0.0003657249,0.00151519,0.001115849],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001153628,"about_ca_system_score_gemma":0.001146408,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004118823,"about_ca_topic_score_gemma":0.00508755,"domain_scores_codex":[0.999279,0.0001760121,0.0001121556,0.0001649404,0.0001709753,0.00009700316],"domain_scores_gemma":[0.9993057,0.0002256887,0.00008620987,0.0001614106,0.0001127093,0.0001082338],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001450812,0.00006535935,0.0002119977,0.00009443762,0.00001317192,0.00004534936,0.000040633,0.0003315084,0.9969392,0.0003508345,0.0001584277,0.001603952],"study_design_scores_gemma":[0.0000131168,0.00003100256,0.0004331117,0.00000476476,0.00001359571,0.00005015813,0.00001249159,0.0006372398,0.9964545,0.00005789623,0.002285151,0.000007131678],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7704998,0.003611948,0.2080659,0.001281566,0.0005176076,0.001111318,0.009034953,0.00206053,0.003816468],"genre_scores_gemma":[0.8059427,0.002881396,0.1659316,0.0003854008,0.00006981113,0.0007040951,0.0138677,0.0009135382,0.009303798],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004118823,"threshold_uncertainty_score":0.008370221,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00927843279670545,"score_gpt":0.2359647527655865,"score_spread":0.2266863199688811,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}