{"id":"W1987712655","doi":"10.1074/jbc.m114.621110","title":"N-Acetylglucosaminidases from CAZy Family GH3 Are Really Glycoside Phosphorylases, Thereby Explaining Their Use of Histidine as an Acid/Base Catalyst in Place of Glutamic Acid","year":2014,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":64,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Glycoside hydrolase; Histidine; Chemistry; Stereochemistry; Biochemistry; Glycosyl; Subfamily; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009313643,0.0005177156,0.0002410574,0.0001597507,0.0002632586,0.0005248842,0.0002354411,0.0003848989,0.001287316],"category_scores_gemma":[0.0001701615,0.0001159985,0.0003370749,0.0004689198,0.0004331628,0.0004985556,0.0002909401,0.0005822834,0.0005497775],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004330105,"about_ca_system_score_gemma":0.0003477547,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002079448,"about_ca_topic_score_gemma":0.002259526,"domain_scores_codex":[0.9998726,0.000008844243,0.00000682987,0.00004036661,0.00004380116,0.00002761383],"domain_scores_gemma":[0.9999092,0.00001027139,0.00003053409,0.00001387822,0.0000207046,0.00001545882],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001871768,0.00001546273,0.003067376,0.0001995668,0.00002008343,0.0002642586,0.00009125723,0.0001001269,0.9745331,0.0008999502,0.0002867102,0.02033485],"study_design_scores_gemma":[0.0000303439,0.0002788022,0.06993769,0.00002542267,0.00006107498,0.005362459,0.0002682567,0.0008505553,0.8746353,0.002207705,0.04630732,0.00003508566],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9494024,0.0122254,0.02509508,0.0004585441,0.0001800888,0.00007838842,0.001403908,0.0003788399,0.01077744],"genre_scores_gemma":[0.9699524,0.003839573,0.01572962,0.0002180682,0.00002751736,0.00003719702,0.002597778,0.00006090512,0.007536881],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002079448,"threshold_uncertainty_score":0.004306495,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02553923369973714,"score_gpt":0.238370343854117,"score_spread":0.2128311101543799,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}