{"id":"W1987736099","doi":"10.1016/j.ab.2008.06.029","title":"Cryptic splice site in the complementary DNA of glucocerebrosidase causes inefficient expression","year":2008,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Lysosomal Storage Disorders Research","field":"Medicine","cited_by":7,"is_retracted":false,"has_abstract":false,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"","keywords":"Complementary DNA; Chinese hamster ovary cell; Reverse transcriptase; Biology; splice; Glucocerebrosidase; Transcription (linguistics); RNA splicing; Molecular biology; Coding region; Alternative splicing; Messenger RNA; DNA; Rapid amplification of cDNA ends; RNA; Gene; Genetics; Molecular cloning; Cell culture","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006058395,0.0005348481,0.00048333,0.0004290714,0.0003109329,0.0006652251,0.0005396566,0.001006014,0.001709755],"category_scores_gemma":[0.0005525211,0.0003990557,0.0005183694,0.0003401912,0.0007642002,0.0001924366,0.0003480165,0.001458971,0.001163461],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008172831,"about_ca_system_score_gemma":0.0007116259,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007351941,"about_ca_topic_score_gemma":0.0009357437,"domain_scores_codex":[0.9993344,0.0001965296,0.0001147085,0.0001200591,0.000144452,0.00008981207],"domain_scores_gemma":[0.999027,0.0003447275,0.0002096663,0.0002071207,0.00005536451,0.0001560298],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002111976,0.0001098892,0.0002285898,0.00003642552,0.00001791107,0.0004338002,0.00002356563,0.0001645792,0.9967605,0.0006211039,0.0001035132,0.001289006],"study_design_scores_gemma":[0.00006789736,0.0002003587,0.001134965,0.00001356867,0.00004366925,0.001217745,0.00002877204,0.001907455,0.9935263,0.0002088279,0.00163619,0.00001421269],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.989076,0.0004837703,0.007691872,0.0002531041,0.000186206,0.00005914778,0.0004504937,0.000211995,0.001587431],"genre_scores_gemma":[0.9949181,0.0002806588,0.002913773,0.0001032056,0.00002977689,0.00002765173,0.0005101624,0.0001197781,0.001096821],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001709755,"threshold_uncertainty_score":0.005929828,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03373453749884782,"score_gpt":0.3172709343336311,"score_spread":0.2835363968347833,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}