{"id":"W1988379604","doi":"10.1016/j.biochi.2009.09.017","title":"Increasing the synthesis/hydrolysis ratio of aminoacylase 1 by site-directed mutagenesis","year":2009,"lang":"en","type":"article","venue":"Biochimie","topic":"Protein Structure and Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute","funders":"","keywords":"Chemistry; Hydrolysis; Acylation; Amino acid; Catalysis; Residue (chemistry); Stereochemistry; Active site; Site-directed mutagenesis; Serine; Enzyme; Mutant; Organic chemistry; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001793496,0.0001578276,0.0001640976,0.00003400414,0.0001046462,0.00002042018,0.0002214753,0.0001387041,0.00001624957],"category_scores_gemma":[0.0002592833,0.0001156806,0.0001162598,0.000141665,0.00008073478,0.000003218645,0.00004627917,0.0000555524,0.000003659552],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009099779,"about_ca_system_score_gemma":0.00004023226,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006761297,"about_ca_topic_score_gemma":0.00001886178,"domain_scores_codex":[0.9990953,0.0001194894,0.0002123536,0.0002550106,0.0001330531,0.0001847513],"domain_scores_gemma":[0.9992839,0.00004041941,0.0001212238,0.0004442279,0.00005619117,0.00005402029],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001001013,0.00003854071,0.0005437706,0.000005516072,0.00004447735,0.000001214269,0.00002053538,0.00002706328,0.9903231,0.00003838011,0.001227258,0.007630042],"study_design_scores_gemma":[0.0001770706,0.0001039146,0.006110663,0.000009585501,0.00007037419,0.00001699557,0.00001498309,0.0004242437,0.9907544,0.00007543012,0.002073537,0.0001688355],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9962226,0.001432585,0.001063225,0.0002534594,0.00004199915,0.0001554833,0.00007980776,0.00002612589,0.0007246936],"genre_scores_gemma":[0.9979073,0.0001201571,0.001165027,0.0003829714,0.000114104,0.00001080588,0.0001268632,0.00001343146,0.0001593381],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007461207,"threshold_uncertainty_score":0.4717319,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004255961315714742,"score_gpt":0.2096210304566995,"score_spread":0.2053650691409848,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}