{"id":"W1988542512","doi":"10.1371/journal.pone.0054966","title":"A New Strategy to Produce a Defensin: Stable Production of Mutated NP-1 in Nitrate Reductase-Deficient Chlorella ellipsoidea","year":2013,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Antimicrobial Peptides and Activities","field":"Immunology and Microbiology","cited_by":49,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institute of Genetics; Peking University; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences; Chinese Academy of Sciences","keywords":"Defensin; Escherichia coli; Nitrate reductase; Biology; Transgene; Electroporation; Gene; Biochemistry; Molecular biology; Heterologous expression; Bacillus subtilis; Chemistry; Recombinant DNA; Genetics; Enzyme; Bacteria","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002048436,0.000494019,0.0003124305,0.0002527979,0.0001924553,0.0002178618,0.0004371327,0.000378694,0.0003453105],"category_scores_gemma":[0.00009359245,0.0001571555,0.0002821987,0.0001560579,0.0002018394,0.0002810147,0.0003970219,0.0008803931,0.0001948689],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000329244,"about_ca_system_score_gemma":0.0002456284,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008230131,"about_ca_topic_score_gemma":0.001044576,"domain_scores_codex":[0.9998667,0.0000164692,0.00001853872,0.00003571777,0.00004700621,0.00001555135],"domain_scores_gemma":[0.9998978,0.00001164025,0.00003168376,0.00001566036,0.00001630981,0.00002683151],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000006260206,0.0000064345,0.0000385427,0.00001939224,0.000001399632,0.00001872747,0.000007613749,0.00001558237,0.9993384,0.00003056655,0.000007245267,0.0005096681],"study_design_scores_gemma":[0.00001143504,0.0001232354,0.001532475,0.000006959106,0.0000149606,0.0003361656,0.00002689785,0.0007315252,0.9946567,0.00004723913,0.002501749,0.00001081626],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9455975,0.001484191,0.04998271,0.0003177453,0.0000591545,0.000173499,0.0005477401,0.000256963,0.001580456],"genre_scores_gemma":[0.9055868,0.001655232,0.08715325,0.0001501287,0.00001005193,0.0001431391,0.001241009,0.00008789609,0.003972599],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008230131,"threshold_uncertainty_score":0.002388835,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0300273354757996,"score_gpt":0.2084045846722558,"score_spread":0.1783772491964562,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}