{"id":"W1988687570","doi":"10.1074/jbc.m112.376202","title":"The ATPase Activity of the P-glycoprotein Drug Pump Is Highly Activated When the N-terminal and Central Regions of the Nucleotide-binding Domains Are Linked Closely Together","year":2012,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Drug Transport and Resistance Mechanisms","field":"Medicine","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Institute of General Medical Sciences; Canadian Institutes of Health Research","keywords":"Transmembrane domain; ATP-binding cassette transporter; Nucleotide; Linker; ATPase; Cyclic nucleotide-binding domain; Chemistry; Binding site; P-glycoprotein; Transmembrane protein; Biochemistry; Biophysics; Plasma protein binding; Stereochemistry; Biology; Transporter; Amino acid; Enzyme; Multiple drug resistance; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001274669,0.0002501314,0.0002607211,0.00009357379,0.000116303,0.0002528016,0.0001048382,0.0002605052,0.0008684343],"category_scores_gemma":[0.00009578914,0.0001313681,0.0002341481,0.0001037672,0.0002227132,0.0002142437,0.0001684295,0.0003663792,0.000230559],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002016188,"about_ca_system_score_gemma":0.0001107844,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001968035,"about_ca_topic_score_gemma":0.0003183876,"domain_scores_codex":[0.9998746,0.00001874181,0.00000960847,0.00003541961,0.00003671988,0.00002485616],"domain_scores_gemma":[0.999936,0.00001456633,0.00002004703,0.000005615187,0.000009045809,0.00001474734],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002291527,0.00000737328,0.0001297716,0.00001260081,0.000003520506,0.00002786455,0.000004359081,0.000009807708,0.9991879,0.00002953463,0.00001238528,0.0005519473],"study_design_scores_gemma":[0.000009712742,0.0001467811,0.005348493,0.00000392024,0.00000923074,0.0003066764,0.00001231676,0.000303974,0.9922544,0.0000468419,0.001554422,0.000003229438],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9948503,0.001912685,0.002009374,0.00008693886,0.00002127712,0.00001255511,0.0001147574,0.00003517921,0.0009568918],"genre_scores_gemma":[0.9973646,0.0006327786,0.001077482,0.00004547857,0.000009254578,0.00001026456,0.0002047446,0.000005896986,0.0006493762],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008684343,"threshold_uncertainty_score":0.00290525,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02080171632010553,"score_gpt":0.241679006712229,"score_spread":0.2208772903921235,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}