{"id":"W1988744799","doi":"10.1371/journal.pone.0018317","title":"Mitochondrial Structure and Function Are Disrupted by Standard Isolation Methods","year":2011,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Mitochondrial Function and Pathology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":318,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; McGill University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Fondation pour la Recherche Médicale","keywords":"Mitochondrion; Cell biology; Biology; Mitochondrial permeability transition pore; ATP–ADP translocase; Mitochondrial membrane transport protein; Organelle; Mitochondrial apoptosis-induced channel; Mitochondrial fission; DNAJA3; Biochemistry; mitochondrial fusion; Mitochondrial DNA; Inner mitochondrial membrane; Apoptosis; Programmed cell death","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006931456,0.0001264904,0.000161757,0.00003252476,0.00007785296,0.00001470904,0.0000549586,0.0002334767,0.0003735107],"category_scores_gemma":[0.0001254447,0.0001193127,0.00003280992,0.00005666599,0.00004995617,0.000006013396,0.0000407537,0.0001039119,0.000005618971],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007348715,"about_ca_system_score_gemma":0.00001579253,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007604117,"about_ca_topic_score_gemma":0.00002149415,"domain_scores_codex":[0.9991448,0.0001490979,0.0001506763,0.0003154207,0.00009852024,0.0001414755],"domain_scores_gemma":[0.9995186,0.000009899309,0.00009224842,0.0002107266,0.0000916129,0.00007695941],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008657197,0.00008879628,0.003377161,0.00001627028,0.0000979506,5.532518e-7,0.00006616715,2.181973e-7,0.9915788,0.00003953594,0.002377652,0.001491193],"study_design_scores_gemma":[0.001034441,0.0009289793,0.00273411,0.00001569779,0.0001531954,0.000006275429,0.00005661812,0.00005416224,0.9833453,0.0004620295,0.01098826,0.0002208932],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8951866,0.0007614258,0.1018931,0.00007876669,0.0006014679,0.0003127406,0.0001573129,0.00003678689,0.0009718313],"genre_scores_gemma":[0.8219091,0.0002521478,0.1718567,0.0007739697,0.001441282,0.00004773539,0.0008453581,0.00006317395,0.002810578],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07327756,"threshold_uncertainty_score":0.4865429,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03216022619665694,"score_gpt":0.2573876133096515,"score_spread":0.2252273871129946,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}