{"id":"W1989046662","doi":"10.1021/bi035224j","title":"Steady-State Kinetics and Molecular Evolution of <i>Escherichia coli</i> MenD [(1<i>R</i>,6<i>R</i>)-2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate Synthase], an Anomalous Thiamin Diphosphate-Dependent Decarboxylase−Carboligase","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Biochemical Acid Research Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":32,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"","keywords":"Carboxylate; Kinetics; ATP synthase; Escherichia coli; Chemistry; Enzyme kinetics; Biochemistry; Enzyme; Physics; Active site; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0005221856,0.0007123601,0.0006241623,0.00008631364,0.0001597823,0.00006705022,0.0005473077,0.0005244231,0.00001799903],"category_scores_gemma":[0.0005056549,0.0007235677,0.0002075123,0.0003543552,0.0007227626,0.0000196118,0.000430426,0.0003836507,0.000004227859],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001343811,"about_ca_system_score_gemma":0.0002573478,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00009173124,"about_ca_topic_score_gemma":0.00001995717,"domain_scores_codex":[0.9960368,0.0002311027,0.0007555722,0.001328968,0.0006489898,0.000998617],"domain_scores_gemma":[0.9974281,0.00004978482,0.0003414236,0.001186648,0.0003519775,0.0006420838],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002911993,0.0004943356,0.001806959,0.0002494011,0.0002448834,0.00005013724,0.00005867888,0.0000581314,0.9962735,0.00007530495,0.0001773121,0.0002201207],"study_design_scores_gemma":[0.001561912,0.0007419942,0.0005208964,0.00006876225,0.0001165353,0.00005099487,0.000217339,0.0001568259,0.9945729,0.0002311502,0.0009882762,0.0007724219],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9829893,0.0134733,0.0005565464,0.00007562699,0.0001390354,0.0004953532,0.0002385937,0.00005996068,0.001972335],"genre_scores_gemma":[0.9966932,0.001016208,0.001338602,0.0001660536,0.0001088341,0.00009172874,0.0001419981,0.0001078782,0.0003355144],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01370393,"threshold_uncertainty_score":0.9995216,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005530617984649116,"score_gpt":0.227842943122002,"score_spread":0.2223123251373529,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}