{"id":"W1989054737","doi":"10.1016/j.ymben.2010.03.002","title":"RCA-I-resistant CHO mutant cells have dysfunctional GnT I and expression of normal GnT I in these mutants enhances sialylation of recombinant erythropoietin","year":2010,"lang":"en","type":"article","venue":"Metabolic Engineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"World Anti-Doping Agency; University of Michigan; Amgen","keywords":"Mutant; Chinese hamster ovary cell; Glycosylation; Complementation; Molecular biology; Recombinant DNA; Erythropoietin; Transfection; Biology; Complementary DNA; Glycoprotein; HEK 293 cells; Glycan; Cell culture; Mutant protein; Wild type; Biochemistry; Chemistry; Gene; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002038869,0.0001575079,0.0002332056,0.0001022307,0.00002980021,0.00001010288,0.00009006048,0.0001266433,0.00005137528],"category_scores_gemma":[0.00009205961,0.0001353786,0.0000763772,0.00008946962,0.00005241235,0.0000122443,0.00008149727,0.0001273923,6.208833e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000475267,"about_ca_system_score_gemma":0.00003409086,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001040988,"about_ca_topic_score_gemma":0.00005959105,"domain_scores_codex":[0.999038,0.00003070523,0.0003514416,0.0002432498,0.0001498847,0.0001866634],"domain_scores_gemma":[0.9994895,0.00002109731,0.0001397379,0.0002156257,0.00006256886,0.00007147049],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001044286,0.00009475236,0.003065007,0.00006042384,0.00001598743,0.000001383013,0.0000911864,0.002858963,0.9928207,0.00006002405,0.0000205741,0.0008065322],"study_design_scores_gemma":[0.0005197303,0.0001115679,0.01656339,0.00006516743,0.0000144821,0.000004113703,0.00004828532,0.00124665,0.9790432,0.00002618704,0.002209786,0.0001474511],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9969477,0.0008647579,0.001217847,0.00000980189,0.0006870967,0.0001694446,0.00002141615,0.000008453077,0.00007350226],"genre_scores_gemma":[0.9987365,0.0003992749,0.0006200729,0.000007313681,0.0001264511,0.00003066747,0.00001645793,0.00001825016,0.00004499131],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01377754,"threshold_uncertainty_score":0.5520577,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003771173861807909,"score_gpt":0.2139475466333857,"score_spread":0.2101763727715778,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}