{"id":"W1989202961","doi":"10.1093/protein/gzt057","title":"Semi-rational approach for converting a GH1  -glycosidase into a  -transglycosidase","year":2013,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":84,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Canadian Glycomics Network; European Synchrotron Radiation Facility","keywords":"Thermus thermophilus; Glycoside hydrolase; Hydrolysis; Mutagenesis; Hydrolase; Chemistry; Enzyme; Mutant; Biochemistry; Site-directed mutagenesis; Directed evolution; Stereochemistry; Active site; Saturated mutagenesis; Gene; Escherichia coli","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003307283,0.0006331173,0.0004345452,0.0002428322,0.0002003137,0.0004614713,0.0005776063,0.0004529972,0.0005952087],"category_scores_gemma":[0.0002525717,0.0002831006,0.0004499193,0.0003142342,0.0003554681,0.0002921918,0.0003676236,0.001089582,0.0004791152],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004065438,"about_ca_system_score_gemma":0.0006069309,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007426753,"about_ca_topic_score_gemma":0.0007830801,"domain_scores_codex":[0.9997688,0.00004622731,0.00002842267,0.00004955864,0.00006968257,0.00003737919],"domain_scores_gemma":[0.9998783,0.00002085729,0.00003649024,0.00001991686,0.00002427615,0.00002007694],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001353934,0.0001775571,0.0002146452,0.0001795574,0.00003505474,0.0001945328,0.00003095659,0.002872917,0.9878547,0.001516283,0.0001208693,0.006667443],"study_design_scores_gemma":[0.0000501455,0.0005979142,0.0004218617,0.00001436419,0.00004477041,0.0003891384,0.00002635762,0.005380433,0.9862797,0.0003552976,0.00642197,0.00001792095],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8067886,0.003467096,0.181638,0.0004863882,0.0001775051,0.0006642828,0.000645749,0.0005743956,0.005557886],"genre_scores_gemma":[0.8626136,0.003879892,0.1282558,0.0001573753,0.00002191259,0.0002607186,0.000763741,0.0001273732,0.00391951],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007426753,"threshold_uncertainty_score":0.002949715,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007205339398512438,"score_gpt":0.1953933184713576,"score_spread":0.1881879790728452,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}